Anti-HECT E3 ubiquitin ligase antibody (ab83853)


  • Product nameAnti-HECT E3 ubiquitin ligase antibody
    See all HECT E3 ubiquitin ligase primary antibodies
  • Description
    Rabbit polyclonal to HECT E3 ubiquitin ligase
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide within residues: FHPEELKDVI VGNTDYDWKT FEKNARYEPG YNSSHPTIVM FWKAFHKLTL , corresponding to internal sequence amino acids 900-949 of Human HECT E3 ubiquitin ligase (NP_057407).

  • Positive control
    • HepG2 cell lysate.


Associated products


Our Abpromise guarantee covers the use of ab83853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 117 kDa (predicted molecular weight: 117 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/100.


  • FunctionMajor E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Makes part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.
  • Tissue specificityExpressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.
  • Sequence similaritiesContains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 5 RCC1 repeats.
  • Post-translational
  • Cellular localizationCytoplasm > perinuclear region. Associated with the polyribosomes, probably via the 60S subunit.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEB1 antibody
    • CEBP1 antibody
    • Cyclin E binding protein 1 antibody
    • Cyclin-E-binding protein 1 antibody
    • E3 ISG15--protein ligase HERC5 antibody
    • HECT domain and RCC1 like domain containing protein 5 antibody
    • HECT domain and RCC1-like domain-containing protein 5 antibody
    • Hect domain and RLD 5 antibody
    • HECT E3 ubiquitin ligase antibody
    • HERC5 antibody
    • HERC5_HUMAN antibody
    • Herc6 antibody
    see all

Anti-HECT E3 ubiquitin ligase antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling HECT E3 ubiquitin ligase with ab83853 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies and processes of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - HECT E3 ubiquitin ligase. Right - Merge.
  • Anti-HECT E3 ubiquitin ligase antibody (ab83853) at 1 µg/ml (5% skim milk / PBS ) + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 117 kDa
    Observed band size : 117 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 6-18%
  • ICC/IF image of ab83853 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83853, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-HECT E3 ubiquitin ligase antibody (ab83853)

ab83853 has not yet been referenced specifically in any publications.

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