Anti-HECT E3 ubiquitin ligase antibody (ab90146)

Overview

  • Product name
    Anti-HECT E3 ubiquitin ligase antibody
    See all HECT E3 ubiquitin ligase primary antibodies
  • Description
    Rabbit polyclonal to HECT E3 ubiquitin ligase
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 288-337 (LVAELVGYRV TQIACGRWHT LAYVSDLGKV FSFGSGKDGQ LGNGGTRDQL) of Human HECT E3 ubiquitin ligase (NP_057407).

  • Positive control
    • HT1080 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 117 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Makes part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant-negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.
  • Tissue specificity
    Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.
  • Sequence similarities
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 5 RCC1 repeats.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localization
    Cytoplasm > perinuclear region. Associated with the polyribosomes, probably via the 60S subunit.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEB1 antibody
    • CEBP1 antibody
    • Cyclin E binding protein 1 antibody
    • Cyclin-E-binding protein 1 antibody
    • E3 ISG15--protein ligase HERC5 antibody
    • HECT domain and RCC1 like domain containing protein 5 antibody
    • HECT domain and RCC1-like domain-containing protein 5 antibody
    • Hect domain and RLD 5 antibody
    • HECT E3 ubiquitin ligase antibody
    • HERC5 antibody
    • HERC5_HUMAN antibody
    • Herc6 antibody
    see all

Images

  • Anti-HECT E3 ubiquitin ligase antibody (ab90146) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 117 kDa

References

ab90146 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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