Anti-HECTD1 antibody (ab101992)
Key features and details
- Rabbit polyclonal to HECTD1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HECTD1 antibody -
Description
Rabbit polyclonal to HECTD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide, corresponding to N terminal amino acids 1-50 of Human HECTD1 (AAP13073.1).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 290 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 290 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. May be required for development of the head mesenchyme and neural tube closure. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the UPL family. K-HECT subfamily.
Contains 4 ANK repeats.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 MIB/HERC2 domain. - Information by UniProt
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Database links
- Entrez Gene: 25831 Human
- Entrez Gene: 207304 Mouse
- Entrez Gene: 362736 Rat
- SwissProt: Q9ULT8 Human
- SwissProt: Q69ZR2 Mouse
- Unigene: 708017 Human
- Unigene: 249391 Mouse
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Alternative names
- E3 ligase for inhibin receptor antibody
- E3 ubiquitin protein ligase HECTD1 antibody
- E3 ubiquitin-protein ligase HECTD1 antibody
see all
Images
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All lanes : Anti-HECTD1 antibody (ab101992) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 290 kDa
Exposure time: 3 minutes -
ab101992 at 1 µg/ml staining HECTD1 in HeLa cell lysate immunoprecipitated using ab101992 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab101992 has been referenced in 6 publications.
- Meng M & Wu YC LMX1B Activated Circular RNA GFRA1 Modulates the Tumorigenic Properties and Immune Escape of Prostate Cancer. J Immunol Res 2022:7375879 (2022). PubMed: 35832649
- Harris LD et al. The deubiquitinase TRABID stabilizes the K29/K48-specific E3 ubiquitin ligase HECTD1. J Biol Chem 296:100246 (2021). PubMed: 33853758
- Saez I et al. Insights into the ubiquitin-proteasome system of human embryonic stem cells. Sci Rep 8:4092 (2018). PubMed: 29511261
- Flack JE et al. Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3 Ubiquitin Ligase Hyd/UBR5. Mol Cell 67:181-193.e5 (2017). PubMed: 28689657
- Byrne R et al. Activity-Based Probes for HECT E3 Ubiquitin Ligases. Chembiochem 18:1415-1427 (2017). PubMed: 28425671
- Loganzo F et al. Tumor cells chronically treated with a trastuzumab-maytansinoid antibody-drug conjugate develop varied resistance mechanisms but respond to alternate treatments. Mol Cancer Ther 14:952-63 (2015). PubMed: 25646013