Overview

  • Product name
  • Description
    Rabbit polyclonal to HECTD1
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to N terminal amino acids 1-50 of Human HECTD1 (AAP13073.1).

  • Positive control
    • HeLa and 293T whole cell lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab101992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 290 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. May be required for development of the head mesenchyme and neural tube closure.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the UPL family. K-HECT subfamily.
    Contains 4 ANK repeats.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 MIB/HERC2 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ligase for inhibin receptor antibody
    • E3 ubiquitin protein ligase HECTD1 antibody
    • E3 ubiquitin-protein ligase HECTD1 antibody
    • EULIR antibody
    • HECD1_HUMAN antibody
    • HECT domain containing protein 1 antibody
    • HECT domain-containing protein 1 antibody
    • HECTD1 antibody
    • KIAA1131 antibody
    see all

Images

  • ab101992 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101992 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-HECTD1 antibody (ab101992) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 290 kDa


    Exposure time : 3 minutes
  • ab101992 at 1 µg/ml staining HECTD1 in HeLa cell lysate immunoprecipitated using ab101992 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes

References

This product has been referenced in:
  • Loganzo F  et al. Tumor cells chronically treated with a trastuzumab-maytansinoid antibody-drug conjugate develop varied resistance mechanisms but respond to alternate treatments. Mol Cancer Ther 14:952-63 (2015). Read more (PubMed: 25646013) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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