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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: PBS, pH 7.2
Concentration information loading...
Purity Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 97 kDa.
Function E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8.
Pathway Protein modification; protein ubiquitination.
Sequence similarities Contains 1 DOC domain. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Cellular localization Cytoplasm > perinuclear region.
Information by UniProt
E3 ubiquitin-protein ligase HECTD3 antibody
Anti-HECTD3 antibody images
Western blot - HECTD3 antibody (ab67985)
All lanes : Anti-HECTD3 antibody (ab67985) at 1/500 dilution Lane 1 : HECTD3 (isoform 3) transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate Lysates/proteins at 25 µg per lane. Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution Predicted band size : 97 kDa Observed band size : 25 kDa ( why is the actual band size different from the predicted?)
References for Anti-HECTD3 antibody (ab67985)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"