Overview

  • Product nameAnti-HECTD3 antibody
    See all HECTD3 primary antibodies
  • Description
    Mouse polyclonal to HECTD3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-210 (isoform 3) of Human HECTD3

  • Positive control
    • HECTD3 transfected mammalian cells lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 97 kDa.

Target

  • FunctionE3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 DOC domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
  • Cellular localizationCytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase HECTD3 antibody
    • HECD3_HUMAN antibody
    • HECT antibody
    • HECT domain containing protein 3 antibody
    • HECT domain-containing protein 3 antibody
    • HECTD3 antibody
    • Probable E3 ubiquitin protein ligase HECTD3 antibody
    see all

Anti-HECTD3 antibody images

  • All lanes : Anti-HECTD3 antibody (ab67985) at 1/500 dilution

    Lane 1 : HECTD3 (isoform 3) transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 97 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

References for Anti-HECTD3 antibody (ab67985)

ab67985 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab67985.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"