antigen sequence, corresponding to amino acids 500-614 of Human HECW1.
Our Abpromise guarantee covers the use of ab121264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform Heat Induced Epitope Retrieval (HIER) at pH 6 using a pressure boiler as heat source.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml. Fix cells with PFA/Triton X-100.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"