• Product name
  • Description
    Rabbit polyclonal to HECW1
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 500-614 of Human HECW1.

  • Positive control
    • Human cerebral cortex tissue; A431 cells.



Our Abpromise guarantee covers the use of ab121264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform Heat Induced Epitope Retrieval (HIER) at pH 6 using a pressure boiler as heat source.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fix cells with PFA/Triton X-100.


  • Function
    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1. Also targets the mutant SOD1 protein involved in familial amyotrophic lateral sclerosis (FALS). Forms cytotoxic aggregates with DVL1, SSR3 and mutant SOD1 that lead to motor neuron death in FALS.
  • Tissue specificity
    Predominantly expressed in neurons of adult and fetal brain. Weakly expressed in the kidney.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 2 WW domains.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • C2 and WW domain-containing protein 1 antibody
    • E3 ubiquitin-protein ligase HECW1 antibody
    • HECT antibody
    • HECT type E3 ubiquitin ligase antibody
    • HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 antibody
    • HECT, C2 and WW domain-containing protein 1 antibody
    • HECW 1 antibody
    • Hecw1 antibody
    • HECW1_HUMAN antibody
    • hNEDL1 antibody
    • NEDD4 like ubiquitin protein ligase 1 antibody
    • NEDD4-like E3 ubiquitin-protein ligase 1 antibody
    • NEDL1 antibody
    see all


  • ab121264, at 1/15 dilution, staining HECW1 in paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry, showing strong nuclear and cytoplasmic positivity in neuronal cells.
  • ab121264, at 4 µg/ml, staining HECW1 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence, showing positivity in nucleus but not nucleoli & cytoplasm.


This product has been referenced in:
  • Pan H  et al. Novel somatic mutations identified by whole-exome sequencing in muscle-invasive transitional cell carcinoma of the bladder. Oncol Lett 11:1486-1492 (2016). Read more (PubMed: 26893765) »

See 1 Publication for this product

Customer reviews and Q&As

Thank very much for your email. First, I would like to apologize as I have no idea why I pasted HAT1 sequence in my email; a totally unrelated antibody because we also take phone calls so maybe there was mix up. I apologize for any inconvenience.

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Thank you for contacting us.
The full sequence of Human HAT1 as follows:
>sp|O14929|HAT1_HUMAN Histone acetyltransferase type B catalytic subunit OS=Homo sapiens GN=HAT1 PE=1 SV=1

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Thank you for contacting us.
The main difference between the two is Immunogen. The immunogen of ab12164 corresponds to amino acid 500-614 and for ab121265 is from aa 356-481.
The other difference is conc. of antibodies.
I am glad to hear...

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