Overview

  • Product nameAnti-HEF1 antibody
    See all HEF1 primary antibodies
  • Description
    Mouse polyclonal to HEF1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human HEF1, amino acids 1-834 (NP_006394.1).

  • Positive control
    • 293 cell lysate, HEF1 transfected 293T lysate, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 93 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionDocking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.
  • Tissue specificityWidely expressed. Higher levels detected in kidney, lung, and placenta. Also detected in T-cells, B-cells and diverse cell lines. The protein has been detected in lymphocytes, in diverse cell lines, and in lung tissues.
  • Sequence similaritiesBelongs to the CAS family.
    Contains 1 SH3 domain.
  • DomainContains a central domain containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif confers specific interaction with the HLH proteins ID2, E12 and E47. It is absolutely required for the induction of pseudohyphal growth in yeast and mediates homodimerization and heterodimerization with p130cas.
    The SH3 domain interacts with two proline-rich regions of focal adhesion kinase.
  • Post-translational
    modifications
    Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. The p65 isoform is poorly detected.
    Focal adhesion kinase 1 phosphorylates the protein at the YDYVHL motif (conserved among all cas proteins). The SRC family kinases (FYN, SRC, LCK and CRK) are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Ligation of either integrin beta-1 or B-cell antigen receptor on tonsillar B-cells and B-cell lines promotes tyrosine phosphorylation and both integrin and BCR-mediated tyrosine phosphorylation requires an intact actin network. In fibroblasts transformation with oncogene v-ABL results in an increase in tyrosine phosphorylation. Transiently phosphorylated following CD3 cross-linking and this phosphorylated form binds to CRK and C3G. A mutant lacking the SH3 domain is phosphorylated upon CD3 cross-linking but not upon integrin beta-1 cross-linking. Tyrosine phosphorylation occurs upon stimulation of the G-protein coupled C1a calcitonin receptor in rabbit. Calcitonin-stimulated tyrosine phosphorylation is mediated by calcium- and protein kinase C-dependent mechanisms and requires the integrity of the actin cytoskeleton.
  • Cellular localizationCytoplasm > cytoskeleton > spindle and Cytoplasm > cell cortex. Nucleus. Golgi apparatus. Cell projection > lamellipodium. Cytoplasm. Cell junction > focal adhesion. Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cas like docking antibody
    • Cas scaffolding protein family member 2 antibody
    • CAS-L antibody
    • CAS2 antibody
    • CasL antibody
    • CASL_HUMAN antibody
    • CASS2 antibody
    • Crk associated substrate related antibody
    • Crk associated substrate related protein antibody
    • CRK-associated substrate-related protein antibody
    • dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)) antibody
    • dJ49G10.2 antibody
    • dJ761I2.1 (enhancer of filamentation (HEF1)) antibody
    • dJ761I2.1 antibody
    • Enhancer of filamentation 1 antibody
    • Enhancer of filamentation 1 p55 antibody
    • HEF 1 antibody
    • HEF1 antibody
    • NEDD-9 antibody
    • Nedd9 antibody
    • Neural precursor cell expressed developmentally down-regulated protein 9 antibody
    • P105 antibody
    • Renal carcinoma antigen NY-REN-12 antibody
    see all

Anti-HEF1 antibody images

  • Anti-HEF1 antibody (ab88584) at 1 µg/ml + 293 cell lysate at 50 µg

    Secondary
    Goat anti-Mouse IgG (H+L) HRP at 1/5000 dilution

    Predicted band size : 93 kDa
    Observed band size : 93 kDa
  • All lanes : Anti-HEF1 antibody (ab88584) at 1 µg/ml

    Lane 1 : HEF1 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H+L) HRP at 1/5000 dilution

    Predicted band size : 93 kDa
    Observed band size : 93 kDa
  • ab88584 at 10µg/ml staining HEF1 in HeLa cells by Immunofluorescence.

References for Anti-HEF1 antibody (ab88584)

ab88584 has not yet been referenced specifically in any publications.

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