Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880)

Overview

  • Product name
    Hela-DFO treated (0.5mM, 24h) Nuclear Lysate
  • General notes
    We recommend aliquoting the extracts into single use fractions and then storing them at -80°C.


    The cells were treated for 24 hours with a 0.5mM solution of Deferoxamine (DFO). DFO is used as a hypoxia-mimetic agent to stabilize Hypoxia Inducible Factor 1 (HIF-1). DFO stabilizes HIF-1 through the inhibition of Prolyl Hydroxylases (PHDs) which target HIF-1 through degradation. The mechanism of DFO inhibition is likely through the chelation of Fe2+ bound to the active site of PHD which is required for enzymatic activity.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: 10% DTT
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Lysate notes
    The cells were treated for 24 hours with a 0.5mM solution of Deferoxamine (DFO). DFO is used as a hypoxia-mimetic agent to stabilize Hypoxia Inducible Factor 1 (HIF-1). DFO stabilizes HIF-1 through the inhibition of Prolyl Hydroxylases (PHDs) which target HIF-1 through degradation. The mechanism of DFO inhibition is likely through the chelation of Fe2+ bound to the active site of PHD which is required for enzymatic activity.
  • Background
    HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

Images

  • All lanes : Simply Blue Coomassie Stain 20ml

    Lane 1 : HeLa nuclear extract lysate (ab150036)
    Lane 2 : Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880)

    Lysates/proteins at 10 µg per lane.
  • All lanes : Anti-HIF-1 alpha antibody [H1alpha67] - ChIP Grade (ab1) at 5 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880)

    Lysates/proteins at 40 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
  • All lanes : Anti-HIF-1 alpha antibody [EP1215Y] (ab51608) at 1/2000 dilution

    Lane 1 : HeLa nuclear extract lysate (ab150036)
    Lane 2 : Hela-DFO treated (0.5mM, 24h) Nuclear Lysate (ab180880)

    Lysates/proteins at 40 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

References

ab180880 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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