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Product was previously marketed under the MitoSciences sub-brand.
The cells were treated for 24 hours with a 0.5mM solution of Deferoxamine (DFO). DFO is used as a hypoxia-mimetic agent to stabilize Hypoxia Inducible Factor 1 (HIF-1). DFO stabilizes HIF-1 through the inhibition of Prolyl Hydroxylases (PHDs) which target HIF-1 through degradation. The mechanism of DFO inhibition is likely through the chelation of Fe2+ bound to the active site of PHD which is required for enzymatic activity.
Our Abpromise guarantee covers the use of ab116322 in the following tested applications.
|WB||Use at an assay dependent concentration.|
ab116322 has not yet been referenced specifically in any publications.