HeLa nuclear extract lysate (2 hr serum response) (ab14650)

Overview

  • Product nameHeLa nuclear extract lysate (2 hr serum response)
    See all HeLa lysates
  • Description
    HeLa nuclear extract lysate
  • General notesWe recommend aliquoting the extracts into single-use fractions and then storing them. Cells were cultured for 24 hours in low serum (0.5%) conditions and then serum stimulated (10%) for 2 hours immediately prior to harvesting.

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • BackgroundHeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

References for HeLa nuclear extract lysate (2 hr serum response) (ab14650)

ab14650 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"