HeLa nuclear extract lysate (ab150036)

Overview

  • Product name
    HeLa nuclear extract lysate
    See all HeLa lysates
  • General notes
    We recommend aliquoting the extracts into single use fractions and then storing them at -80°C.


    This HeLa Nuclear Extract was prepared using a standard nuclear lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a non-reducing lithium dodecyl sulfate sample loading buffer (LDS).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.50
    Constituent: 10% DTT
    Note: Note: This lysate was normalised with SDS-PAGE sample buffer to current concentration.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Lysate notes
    This HeLa Nuclear Extract was prepared using a standard nuclear lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a non-reducing lithium dodecyl sulfate sample loading buffer (LDS).
  • Background
    HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

Images

  • All lanes  stained with Optiblot Blue (ab119211) (20 ml)

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)

    Lysates/proteins at 10 µg per lane.
  • All lanes : Anti-HDAC1 antibody (ab19845) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
  • All lanes : Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (ab63766) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
  • All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
  • All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 51 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

References

ab150036 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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