HeLa nuclear extract lysate (TPA stimulated 2 hr) (ab14653)

Overview

  • Product name
    HeLa nuclear extract lysate (TPA stimulated 2 hr)
    See all HeLa lysates
  • Description
    HeLa nuclear extract lysate
  • General notes
    Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12 myristate, 13 acetate) for two hours at 37°C immediately prior to harvesting. We recommend aliquoting the extracts into single use fractions and then storing.
  • Tested applications
    Suitable for: WB, EMSAmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research areas
  • Background
    HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

Applications

Our Abpromise guarantee covers the use of ab14653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
EMSA Use at an assay dependent dilution.

References

ab14653 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Unfortunately we do not sell the positive control for ab5498, the source of ab5498 has used Jurkat cells treated with a mouse (monoclonal) anti-human Fas (unfortunately not supplied by ourselves, 0.5 µg/mL, 3 hours) or TNF-a to induce cleavage; the lab...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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