Anti-Helicobacter pylori antibody [51-13] (ab8081)

Overview

  • Product nameAnti-Helicobacter pylori antibody [51-13]
    See all Helicobacter pylori primary antibodies
  • Description
    Mouse monoclonal [51-13] to Helicobacter pylori
  • SpecificityThe antibody reacts specifically with Helicobacter bacteria. It does not react with other members of the Enterobacteriaceae.
  • Tested applicationsSuitable for: ELISA, ICC/IFmore details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.1% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone number51-13
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC/IF
  • Application notesThe antibody is reactive with helicobacter using ELISA and fluorescence on bacteria fixed on a glass slide.

    For use in immunofluorescence the antibody should be used undiluted, for ELISA dilute 10x. For immunofluorescence on bacteria solution, bacteria are spread on a slide and dried. Fix bacteria by 3 fast strokes through gas flame. Incubate with monoclonal, wash and incubate with appropriate fluorescent conjugate. For ELISA prepare bacteria suspension in PBS (5x108 bact./ml). Coat ELISA plate with 100 µl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density.
  • Target

    • RelevanceThe spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcimona and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. More recently this bacterium has also been implicated with a number of vascular disorders including heart disease. It is not clear how H. pylori is transmitted or why some patients become symptomatic while others do not. The bacteria are most likely spread from person to person through fecal-to-oral or oral-to-oral routes. Possible environmental reservoirs include contaminated water sources. Serological tests that measure specific H. pylori IgG antibodies can determine if a person has been infected although these methods do have drawbacks and limitations.
    • Alternative names
      • H. pylori antibody

    References for Anti-Helicobacter pylori antibody [51-13] (ab8081)

    ab8081 has not yet been referenced specifically in any publications.

    Product Wall

    The immunogen recognizes a sulphase antigen. The location is unknown.

    Regarding the sensitivity of ab8081 in ELISA, you need a bacteria suspension of approx. 5*10E8 bacteria/ml. Pleae find below a suggested ELISA protocol:- For ELISA dilute antibody 10x. Prepare bacteria suspension in PBS (5x108 bact./ml). Coat ELISA ...

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    This antibody will cross react with other Helicobacter species. Please note that this anitbody is a monoclonal not a rabbit polyclonal, there was a mistake on the datasheet for which we apologise.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"