Anti-Helicobacter pylori antibody [BDI586] (ab20643)

Overview

  • Product name
    Anti-Helicobacter pylori antibody [BDI586]
    See all Helicobacter pylori primary antibodies
  • Description
    Mouse monoclonal [BDI586] to Helicobacter pylori
  • Tested applications
    Suitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Recognises Helicobacter pylori. Does not react in ELISA with C. jejuni, E. coli (mix), Salmonella (mix), Shigella (mix), P. aeruginosa, Yersinia and Citrobacter. Not yet tested in other species.
  • Immunogen

    H. pylori (whole organism).

  • General notes


    Reactive with the 58kDa (Hsp) of H. pylori.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    >90% pure.
  • Primary antibody notes
    Reactive with the 58kDa (Hsp) of H. pylori.
  • Clonality
    Monoclonal
  • Clone number
    BDI586
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20 - 1/200.
WB 1/10 - 1/50. Detects a band of approximately 58 kDa.

Dilution optimised using Chromogenic detection.

ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcimona and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. More recently this bacterium has also been implicated with a number of vascular disorders including heart disease. It is not clear how H. pylori is transmitted or why some patients become symptomatic while others do not. The bacteria are most likely spread from person to person through fecal-to-oral or oral-to-oral routes. Possible environmental reservoirs include contaminated water sources. Serological tests that measure specific H. pylori IgG antibodies can determine if a person has been infected although these methods do have drawbacks and limitations.
  • Alternative names
    • H. pylori antibody

References for Anti-Helicobacter pylori antibody [BDI586] (ab20643)

ab20643 has not yet been referenced specifically in any publications.

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Thank you for contacting Abcam. I believe that the staining that you wish to do would work, however by using a mouse primary antibody on mouse tissue, you do run the risk of having high background. I have attached the Abcam protocol for mouse on mouse ...

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