Anti-Helicobacter pylori antibody [BDI586] (ab20643)

Overview

  • Product nameAnti-Helicobacter pylori antibody [BDI586]
    See all Helicobacter pylori primary antibodies
  • Description
    Mouse monoclonal [BDI586] to Helicobacter pylori
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Recognises Helicobacter pylori. Does not react in ELISA with C. jejuni, E. coli (mix), Salmonella (mix), Shigella (mix), P. aeruginosa, Yersinia and Citrobacter. Not yet tested in other species.
  • Immunogen

    H. pylori (whole organism).

  • General notes


    Reactive with the 58kDa (Hsp) of H. pylori.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notes>90% pure.
  • Primary antibody notesReactive with the 58kDa (Hsp) of H. pylori.
  • ClonalityMonoclonal
  • Clone numberBDI586
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20 - 1/200.
WB 1/10 - 1/50. Detects a band of approximately 58 kDa.

Dilution optimised using Chromogenic detection.

ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceThe spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcimona and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. More recently this bacterium has also been implicated with a number of vascular disorders including heart disease. It is not clear how H. pylori is transmitted or why some patients become symptomatic while others do not. The bacteria are most likely spread from person to person through fecal-to-oral or oral-to-oral routes. Possible environmental reservoirs include contaminated water sources. Serological tests that measure specific H. pylori IgG antibodies can determine if a person has been infected although these methods do have drawbacks and limitations.
  • Alternative names
    • H. pylori antibody

References for Anti-Helicobacter pylori antibody [BDI586] (ab20643)

ab20643 has not yet been referenced specifically in any publications.

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