Helicobacter pylori antibody (HRP) (ab20629)

Product Name 

Helicobacter pylori antibody (HRP)

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Helicobacter pylori (HRP)

Immunogen 

Tissue / cell preparation (ATCC strain 43504).

Species Reactivity 

Has not been absorbed and may react with related microorganisms.

Tested applications 
(see key)

ELISA, IHC-FoFr

Abreviews 

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Application notes 
(see key)

Recommended dilutions
ELISA: 1/20 - 1/1000.
IHC-F: 1/20 - 1/200.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Helicobacter

Relevance 

The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcimona and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. More recently this bacterium has also been implicated with a number of vascular disorders including heart disease. It is not clear how H. pylori is transmitted or why some patients become symptomatic while others do not. The bacteria are most likely spread from person to person through fecal-to-oral or oral-to-oral routes. Possible environmental reservoirs include contaminated water sources. Serological tests that measure specific H. pylori IgG antibodies can determine if a person has been infected although these methods do have drawbacks and limitations.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Conjugation notes 

Estimated molar HRP:IgG substitution is 2-3.

Storage buffer 

Preservative: 0.002% Thimerosal (merthiolate). Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Constituents: PBS, 10mg/ml BSA

Material safety datasheet (MSDS) for this product:
Thimerosal MSDS

Purification notes 

Source: whole cell lysate. Protein A chromatography purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent.

Form 

Liquid

Conjugation 

Horse Radish Peroxidase

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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