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Products:Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Helicobacter
Overview
Product name
Anti-Helicobacter pylori antibody (HRP)
See all Helicobacter pylori products (13) ...
Description
Rabbit polyclonal to Helicobacter pylori (HRP)
Conjugation
HRP
Conjugation notes
Estimated molar HRP:IgG substitution is 2-3.
Tested applications
IHC-FoFr, ELISAmore details
Cross reactivity
Has not been absorbed and may react with related microorganisms.
Immunogen
Tissue / cell preparation (ATCC strain 43504).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.002% Thimerosal (merthiolate). Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
Constituents: PBS, 10mg/ml BSA
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
Source: whole cell lysate. Protein A chromatography purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent.
Clonality
Polyclonal
Isotype
IgG
Research areas
Microbiology >> Organism >> Bacteria >> Gram negative Bacteria >> Helicobacter
Applications
Show applications key
Our Abpromise guarantee covers the use of ab20629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr
ELISA
Application notes
ELISA: 1/20 - 1/1000.
IHC-F: 1/20 - 1/200.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. H. pylori is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcimona and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. More recently this bacterium has also been implicated with a number of vascular disorders including heart disease. It is not clear how H. pylori is transmitted or why some patients become symptomatic while others do not. The bacteria are most likely spread from person to person through fecal-to-oral or oral-to-oral routes. Possible environmental reservoirs include contaminated water sources. Serological tests that measure specific H. pylori IgG antibodies can determine if a person has been infected although these methods do have drawbacks and limitations.
Alternative names
- H. pylori antibody
References for Anti-Helicobacter pylori antibody (HRP) (ab20629)
ab20629 has not yet been referenced specifically in any publications.
Publishing research using ab20629? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"