The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).
Hellethionins belong to a family of plant thionins, and are small-sized multiple-cysteine peptides that are involved in the plant defence against pathogens. The antimicrobial activity and cytotoxicity of thionins have led to the development of two potential applications of thionins: in agriculture transgenic plants containing thionin genes can enhance pathogen resistance, and targeting of thionins by tumor-specific antibodies may support antitumor therapy. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.
Hellethionin D antibody
Western blot - hellethionin antibody (ab65405)
All lanes : Anti-hellethionin antibody (ab65405) at 1/500 dilution
Lane 1 : extracted hellethionins B and C Lane 2 : extracted hellethionin D
Developed using the ECL technique.
Predicted band size: 5 kDa Observed band size: 5 kDa Additional bands at: 10 kDa (possible dimer)