Overview

  • Product name
    Anti-hellethionin antibody
  • Description
    Rabbit polyclonal to hellethionin
  • Specificity
    This antibody reacts with hellethionins A,B,C,D, E and G from H. purpurascens.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Helleborus purpurascens
  • Immunogen

    Hellethionins A, B, C, D, E and G from Helleborus purpurascens, conjugated to ovalbumin

  • Positive control
    • Extracted hellethionins B, C and D

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).
ELISA 1/256000.

Target

  • Relevance
    Hellethionins belong to a family of plant thionins, and are small-sized multiple-cysteine peptides that are involved in the plant defence against pathogens. The antimicrobial activity and cytotoxicity of thionins have led to the development of two potential applications of thionins: in agriculture transgenic plants containing thionin genes can enhance pathogen resistance, and targeting of thionins by tumor-specific antibodies may support antitumor therapy. They seem to exert their toxic effect at the level of the cell membrane. Their precise function is not known.
  • Cellular localization
    Secreted
  • Alternative names
    • Hellethionin D antibody

Images

  • All lanes : Anti-hellethionin antibody (ab65405) at 1/500 dilution

    Lane 1 : extracted hellethionins B and C
    Lane 2 : extracted hellethionin D

    Developed using the ECL technique

    Predicted band size : 5 kDa
    Observed band size : 5 kDa
    Additional bands at : 10 kDa (possible dimer).

References

ab65405 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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