Overview

  • Product name
  • Description
    Rabbit polyclonal to HELT
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse HELT.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse brain; Mouse testis; Mouse heart; Human heart.

Properties

Applications

Our Abpromise guarantee covers the use of ab101842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 27 kDa).

Target

Images

  • All lanes : Anti-HELT antibody (ab101842) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 27 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutesPlease note the current predicted molecular weight for this protein is 27kDa in Mouse and 36kDa in Human.

References

This product has been referenced in:
  • Wende CZ  et al. Hairy/Enhancer-of-Split MEGANE and Proneural MASH1 Factors Cooperate Synergistically in Midbrain GABAergic Neurogenesis. PLoS One 10:e0127681 (2015). WB . Read more (PubMed: 25993409) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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