Overview

  • Product nameAnti-HELZ antibody
    See all HELZ primary antibodies
  • Description
    Mouse monoclonal to HELZ
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment: MEDRRAEKSC EQACESLKRQ DYEMALKHCT EALLSLGQYS MADFTGPCPL EIERIKIESL LYRIASFLQL KNYVQADEDC RHVLGEGLAK GEDAFRAVLC , corresponding to amino acids 1-101 of Human HELZ, NP_055692, with a 26kDa tag.

  • Positive control
    • HeLa cells and HeLa cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab77645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 75, 240 kDa (predicted molecular weight: 219 kDa).
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged HELZ is approximately 0.03ng/ml as a capture antibody.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • RelevanceHELZ (Helicase with zinc finger) belongs to the superfamily I class of RNA helicases. Alteration of RNA secondary structure may subsequently influence interactions with proteins or other nucleic acids.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • DKFZp586G1924 antibody
    • Down regulated in human cancers antibody
    • DRHC antibody
    • Helicase with zinc finger antibody
    • HUMORF 5 antibody
    • HUMORF5 antibody
    • KIAA0054 antibody
    • MGC163454 antibody
    • Probable helicase with zinc finger domain antibody
    see all

Anti-HELZ antibody images

  • Anti-HELZ antibody (ab77645) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 219 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-HELZ antibody (ab77645) at 5 µg/ml + HeLa cell lysate (nuclear) at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 219 kDa
    Observed band size : 219 kDa
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
  • ab77645 at 10µg/ml staining HELZ in Hela cells.
  • Detection limit for this product is ~0.03ng/ml as a capture antibody.

References for Anti-HELZ antibody (ab77645)

ab77645 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"