The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/70000. The anti HA diluted 1/70,000 gave an OD=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification.
Use at an assay dependent dilution.
1/5000. Predicted molecular weight: 1 kDa.
RelevanceHemagglutinin (HA) is a class I viral fusion protein from Influenza virus. It is a major glycoprotein, comprising over 80% of the envelope proteins present in the virus particle. HA binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell, and is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. The extent of infection into host organism is determined by HA. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoproteases secreted by the bronchial epithelial cells. The HA protein is a homotrimer of disulfide-linked HA1-HA2. It also plays a major role in the determination of host range restriction and virulence. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains.
Cellular localizationCell membrane; apical cell membrane; single-pass type I membrane protein.
Anti-Hemagglutinin antibody images
Immunocytochemistry/ Immunofluorescence - Anti-Hemagglutinin antibody (ab20084)Image from Wu CY et al., PLoS One. 2010 Mar 22;5(3):e9784. doi: 10.1371/journal.pone.0009784.; Fig 1.; March 22, 2010, PLoS ONE 5(3): e9784.
Immunofluorescence analysis of influenza virus-like particle producer cells, staining Hemagglutinin (red) with ab20084.
Cells were fixed in paraformaldehyde for 10 min and permeabilized in 0.05% Triton-X 100 for 1 min. After blocking with 1% gelatin, the cells were incubated with primary antibody, followed by incubation with Cy3-conjugated goat anti-rabbit IgG as the secondary antibody.
References for Anti-Hemagglutinin antibody (ab20084)