Overview

  • Product name
    Anti-Hemagglutinin peptide antibody [HA.C5]
  • Description
    Mouse monoclonal [HA.C5] to Hemagglutinin peptide
  • Specificity
    ab59076 is an HA-tag monoclonal antibody. It recognizes HA-tagged proteins overpressed in cells, including both amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Influenza A virus
  • Immunogen

    A synthetic peptide from influenza hemagglutinin epitope (

    YPYDVPDYA

    ) coupled to KLH (HA-Tagged).

  • Positive control
    • 293 cells transfected with HA-tagged vector
  • General notes
    Stable for 1 year at -20°C

Properties

Applications

Our Abpromise guarantee covers the use of ab59076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 63 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
IP 1/200.
ICC/IF 1/200.

Target

  • Relevance
    Hemagglutinin is an antigenic glycoprotein found on the surface of the influenza viruses, as well as many other bacteria and viruses. It is responsible for binding the virus to the cell that is being infected. The name hemagglutinin comes from the protein's ability to cause red blood cells (erythrocytes) to agglutinate in vitro.
  • Cellular localization
    Apical Cell Membrane
  • Alternative names
    • ha antibody

Anti-Hemagglutinin peptide antibody [HA.C5] images

  • All lanes : Anti-Hemagglutinin peptide antibody [HA.C5] (ab59076) at 1/1000 dilution

    Lane 1 : 293 cells, untransfected control
    Lane 2 : 293 cells, transfected with HA-tagged vector


    Predicted band size : 63 kDa
  • Immunofluorescence staining of HA-Tag fusion protein (transcription factor) using ab59076 at a 1/200 dilution.
    Left image (A): control cell line.
    Right image (B): stable expressing cell line.

References for Anti-Hemagglutinin peptide antibody [HA.C5] (ab59076)

This product has been referenced in:
  • Colle MA  et al. Efficient intracerebral delivery of AAV5 vector encoding human ARSA in non-human primate. Hum Mol Genet 19:147-58 (2010). ELISA . Read more (PubMed: 19837699) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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