Overview

  • Product name
    Anti-Heme Oxygenase 1 antibody
    See all Heme Oxygenase 1 primary antibodies
  • Description
    Rabbit polyclonal to Heme Oxygenase 1
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Multi-antigenic synthetic peptide from Human Heme Oxygenase 1

  • Positive control
    • Lysate from heat shocked HeLa cells

Applications

Our Abpromise guarantee covers the use of ab79854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IHC-P 1/50.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.

Target

  • Function
    Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
  • Sequence similarities
    Belongs to the heme oxygenase family.
  • Cellular localization
    Microsome. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • 32 kD antibody
    • bK286B10 antibody
    • D8Wsu38e antibody
    • heat shock protein 32 kD antibody
    • heat shock protein 32kD antibody
    • Heat shock protein antibody
    • Heme oxygenase (decycling) 1 antibody
    • Heme oxygenase 1 antibody
    • Hemox antibody
    • HMOX 1 antibody
    • Hmox antibody
    • Hmox1 antibody
    • HMOX1_HUMAN antibody
    • HO 1 antibody
    • HO antibody
    • HO-1 antibody
    • HO1 antibody
    • Hsp32 antibody
    see all

Anti-Heme Oxygenase 1 antibody images

  • All lanes : Anti-Heme Oxygenase 1 antibody (ab79854) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Cell lysates prepared from human A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G cells


    Predicted band size : 33 kDa
  • Immunocytochemistry/Immunofluorescence analysis of heat shocked HeLa cells using ab79854 at 1/100 dilution for 12 hours at 4°C. Fixation: 2% Formaldehyde for 20 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum membrane. Cytoplasm. Magnification: 100x. Heat Shocked at 42°C for 1h.

References for Anti-Heme Oxygenase 1 antibody (ab79854)

This product has been referenced in:
  • Ni W  et al. Role of Erythrocyte CD47 in Intracerebral Hematoma Clearance. Stroke 47:505-11 (2016). WB, IHC-FoFr ; Mouse . Read more (PubMed: 26732568) »
  • Wang X  et al. Activation of the Nuclear Factor E2-Related Factor 2/Antioxidant Response Element Pathway is Neuroprotective Following Spinal Cord Injury. J Neurotrauma : (2011). WB ; Rat . Read more (PubMed: 21806470) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (brain microsomes spleen cytosol)
Loading amount
75 µg
Specification
brain microsomes spleen cytosol
Treatment
hyperoxia (>95%O2) for 18hrs
Gel Running Conditions
Reduced Denaturing (gel 12,5%)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Sandra Sobocanec

Verified customer

Submitted Oct 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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