within Human Heme Oxygenase 1 aa 1-100 (N terminal). The exact sequence is proprietary. Database link: P09601
HepG2, A549, rat kidney, rat spleen, mouse kidney cell lysate.
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250.
1/10000 - 1/50000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
Use at an assay dependent concentration.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notesIs unsuitable for IHC-P.
FunctionHeme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
Sequence similaritiesBelongs to the heme oxygenase family.
Western blot - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/1000 dilution
Lane 1 : Hek293 Lane 2 : HL60 Lane 3 : HeLa Lane 4 : A549 Lane 5 : Hu spleen Lane 6 : Ms spleen Lane 7 : Rt spleen
Lysates/proteins at 10 µg per lane.
Secondary IRDye® 800CW Goat anti Rabbit
Predicted band size : 33 kDa
Hek293 & HL60 presumed negative or very low expression.
Loading control GAPDH at 38kDa
Immunocytochemistry/ Immunofluorescence - Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)This image is courtesy of an anonymous Abreview
ab68477 (unpurified) staining Heme Oxygenase 1 in mouse embryonic fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1.5% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/100 in 1X HBSS + 0.02% Triton X-100 + 1.5% FBS) for 3 hours at 25°C. An Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Heme Oxygenase 1 with purified ab68477 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Flow cytometry analysis of A549 (human lung carcinoma) cells labeling with purified ab68477 at 1/200 dilution ( 1ug/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077) )(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
Predicted band size : 33 kDa Observed band size : 33 kDa
Blocking and dilution buffer: 5% NFDM/TBST
Western blot - Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
All lanes : Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477) at 1/2000 dilution (purified)
Lane 1 : HepG2 cell lysate Lane 2 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary HRP conjugated Goat anti-Rabbit at 1/2000 dilution
Predicted band size : 33 kDa Observed band size : 33 kDa Additional bands at : 125-130 kDa,30 kDa,48 kDa,52 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Heme Oxygenase 1 antibody [EPR1390Y] (ab68477)
This product has been referenced in:
Shen C et al. Resveratrol pretreatment attenuates injury and promotes proliferation of neural stem cells following oxygen-glucose deprivation/reoxygenation by upregulating the expression of Nrf2, HO-1 and NQO1 in vitro. Mol Med Rep14:3646-54 (2016).
Read more (PubMed: 27573874) »