Anti-Heme Oxygenase 1 antibody [HO-1-2] (FITC) (ab69545)

Overview

  • Product nameAnti-Heme Oxygenase 1 antibody [HO-1-2] (FITC)
    See all Heme Oxygenase 1 primary antibodies
  • Description
    Mouse monoclonal [HO-1-2] to Heme Oxygenase 1 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Hamster, Dog, Human, Monkey
  • Immunogen

    Full length native protein (purified) (Rat)

  • Positive control
    • Jurkat cells

Applications

Our Abpromise guarantee covers the use of ab69545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 100 µg/ml. ab91368-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionHeme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
  • Sequence similaritiesBelongs to the heme oxygenase family.
  • Cellular localizationMicrosome. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • 32 kD antibody
    • bK286B10 antibody
    • D8Wsu38e antibody
    • heat shock protein 32 kD antibody
    • heat shock protein 32kD antibody
    • Heat shock protein antibody
    • Heme oxygenase (decycling) 1 antibody
    • Heme oxygenase 1 antibody
    • Hemox antibody
    • HMOX 1 antibody
    • Hmox antibody
    • Hmox1 antibody
    • HMOX1_HUMAN antibody
    • HO 1 antibody
    • HO antibody
    • HO-1 antibody
    • HO1 antibody
    • Hsp32 antibody
    see all

Anti-Heme Oxygenase 1 antibody [HO-1-2] (FITC) images

  • Flow Cytometry of 106 Jurkat cells stained using ab69545 at a concentration of 100 µg/mL.

References for Anti-Heme Oxygenase 1 antibody [HO-1-2] (FITC) (ab69545)

This product has been referenced in:
  • Chiang N  et al. Inhaled carbon monoxide accelerates resolution of inflammation via unique proresolving mediator-heme oxygenase-1 circuits. J Immunol 190:6378-88 (2013). Read more (PubMed: 23650615) »
  • Walther M  et al. HMOX1 gene promoter alleles and high HO-1 levels are associated with severe malaria in Gambian children. PLoS Pathog 8:e1002579 (2012). Flow Cyt ; Human . Read more (PubMed: 22438807) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (hepatocytes)
Permeabilization Yes - 0.1% Triton X-100
Specification hepatocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative Paraformaldehyde
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Verified customer

Submitted Dec 22 2015

Thank you very much for contacting us.

As discussed please attach the protocol and send it to us, as per your earlier conveniance.

Many thanks!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"