Overview

  • Product name
    Anti-Heme oxygenase 2 antibody
    See all Heme oxygenase 2 primary antibodies
  • Description
    Rabbit polyclonal to Heme oxygenase 2
  • Tested applications
    Suitable for: WB, IP, ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Dog, Human, Pig, Monkey
  • Immunogen

    Synthetic peptide derived from a sequence near the amino-terminus of Human Heme oxygenase 2.

  • Positive control
    • MCF7 cells, human breast cancer tissue, Human, mouse, Rat and Dog Liver Microsome Extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab90515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 36 kDa.
IP Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
  • Sequence similarities
    Belongs to the heme oxygenase family.
    Contains 2 HRM (heme regulatory motif) repeats.
  • Cellular localization
    Microsome. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heme oxygenase (decycling) 2 antibody
    • Heme oxygenase (decyclizing) 2 antibody
    • Heme oxygenase 2 antibody
    • HMOX 2 antibody
    • Hmox2 antibody
    • HMOX2 protein antibody
    • HMOX2_HUMAN antibody
    • HO 2 antibody
    • HO-2 antibody
    • HO2 antibody
    • OTTHUMP00000159847 antibody
    see all

Anti-Heme oxygenase 2 antibody images

  • ab90515 staining Heme oxygenase 2 in MCF7 cells by Immunofluorescence (green). Nuclei are stained in blue pseudocolor using DRAQ5.
  • All lanes : Anti-Heme oxygenase 2 antibody (ab90515) at 1/1000 dilution

    Lane 1 : Heme oxygenase 2 Recombinant Human Protein
    Lane 2 : Human Liver Microsome Extract
    Lane 3 : Mouse Liver Microsome Extract
    Lane 4 : Rat Liver Microsome Extract
    Lane 5 : Dog Liver Microsome Extract


    Predicted band size : 36 kDa

References for Anti-Heme oxygenase 2 antibody (ab90515)

This product has been referenced in:
  • Yang F  et al. Suppression of TRPV4 channels ameliorates anti-dipsogenic effects under hypoxia in the subfornical organ of rats. Sci Rep 6:30168 (2016). WB . Read more (PubMed: 27436489) »
  • Lee S  et al. Chronic Stress Decreases Cerebrovascular Responses During Rat Hindlimb Electrical Stimulation. Front Neurosci 9:462 (2015). WB ; Rat . Read more (PubMed: 26778944) »

See all 2 Publications for this product

Product Wall

Application
Western blot
Sample
Human Tissue lysate - whole (Hep G2, liver cancer)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Hep G2, liver cancer
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 19 2016

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Hep G2)
Specification
Hep G2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 29 2014

Following up my message from a week ago regarding ab90515, Anti-Heme oxygenase 2 antibody, the immunogen, though we were not able to obtain the exact sequence, is within the first 50 amino acids of Human HO-2.

I hope this helps. If you have ...

Read More

Thank you for your interest in this HO-2 antibody. I am waiting for our source to send the immunogen information, which I will forward.

We do not have a non-rabbit/mouse hosted antibody that has been tested for IHC but this otherwise appears...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (lung supernatant)
Loading amount
85 µg
Specification
lung supernatant
Treatment
hyperoxia for 18 hrs
Gel Running Conditions
Reduced Denaturing (12,5%)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Sandra Sobocanec

Verified customer

Submitted May 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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