Anti-Heme oxygenase 2 antibody (ab90515)

Overview

  • Product nameAnti-Heme oxygenase 2 antibody
    See all Heme oxygenase 2 primary antibodies
  • Description
    Rabbit polyclonal to Heme oxygenase 2
  • Tested applicationsSuitable for: WB, IP, ELISA, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Dog, Human, Pig, Monkey
  • Immunogen

    Synthetic peptide derived from a sequence near the amino-terminus of Human Heme oxygenase 2.

  • Positive control
    • MCF7 cells, human breast cancer tissue, Human, mouse, Rat and Dog Liver Microsome Extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab90515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 36 kDa.
IP Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionHeme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
  • Sequence similaritiesBelongs to the heme oxygenase family.
    Contains 2 HRM (heme regulatory motif) repeats.
  • Cellular localizationMicrosome. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heme oxygenase (decycling) 2 antibody
    • Heme oxygenase (decyclizing) 2 antibody
    • Heme oxygenase 2 antibody
    • HMOX 2 antibody
    • Hmox2 antibody
    • HMOX2 protein antibody
    • HMOX2_HUMAN antibody
    • HO 2 antibody
    • HO-2 antibody
    • HO2 antibody
    • OTTHUMP00000159847 antibody
    see all

Anti-Heme oxygenase 2 antibody images

  • ab90515 staining Heme oxygenase 2 in MCF7 cells by Immunofluorescence (green). Nuclei are stained in blue pseudocolor using DRAQ5.
  • All lanes : Anti-Heme oxygenase 2 antibody (ab90515) at 1/1000 dilution

    Lane 1 : Heme oxygenase 2 Recombinant Human Protein
    Lane 2 : Human Liver Microsome Extract
    Lane 3 : Mouse Liver Microsome Extract
    Lane 4 : Rat Liver Microsome Extract
    Lane 5 : Dog Liver Microsome Extract


    Predicted band size : 36 kDa

References for Anti-Heme oxygenase 2 antibody (ab90515)

ab90515 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (Hep G2, liver cancer)
Gel Running Conditions Reduced Denaturing
Loading amount 25 µg
Specification Hep G2, liver cancer
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 19 2016

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Hep G2)
Specification Hep G2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 29 2014

Following up my message from a week ago regarding ab90515, Anti-Heme oxygenase 2 antibody, the immunogen, though we were not able to obtain the exact sequence, is within the first 50 amino acids of Human HO-2.

I hope this helps. If you have ...

Read More

Thank you for your interest in this HO-2 antibody. I am waiting for our source to send the immunogen information, which I will forward.

We do not have a non-rabbit/mouse hosted antibody that has been tested for IHC but this otherwise appears...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (lung supernatant)
Loading amount 85 µg
Specification lung supernatant
Treatment hyperoxia for 18 hrs
Gel Running Conditions Reduced Denaturing (12,5%)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Sandra Sobocanec

Verified customer

Submitted May 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"