• Product nameHemin Assay Kit
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Assay time
    0h 30m
  • Product overview

    Hemin Assay Kit (ab65332) utilizes peroxidase activity in the presence of hemin to provide a simple, exquisitely sensitive assay which causes the conversion of a colorless probe to a strongly colored (? = 570) compound. Trace amounts of Hemin can be quantitated in the 5-160 pg (10-250 fmol) range.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Free hemin results from the breakdown of hemin-containing proteins such as hemoglobin and myoglobin. It can be detected in various body fluids such as saliva, urine and csf under various pathological states. Free hemin exists in cells at a very minute concentrations (< 1µM ˜ 650 ng/ml) exerting regulatory functions such as repression of nonspecific d-aminolevulinate synthase expression and induction of microsomal hemin oxygenase-1. Hemin can stimulate growth of oral bacteria involved in gingivitis and is an indicator of possible pathological conditions when found in the urine or feces.

  • Tested applicationsSuitable for: Functional Studiesmore details


  • RelevanceFree hemin results from the breakdown of hemin-containing proteins such as hemoglobin and myoglobin. Hemin level has been used as an indicator of possible pathological conditions when found in the urine or faeces. It plays regulatory functions for enzyme activities.


Our Abpromise guarantee covers the use of ab65332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Hemin Assay Kit images

  • Hemin measured in various biofluids showing concentration (nM)

  • A: Time course of development of color and B: Standard Curve at 30 minutes for Hemin standard.


References for Hemin Assay Kit (ab65332)

This product has been referenced in:
  • Urasaki Y  et al. Uridine affects liver protein glycosylation, insulin signaling, and heme biosynthesis. PLoS One 9:e99728 (2014). Functional Studies . Read more (PubMed: 24918436) »

See 1 Publication for this product

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The component should be stored as follows;

Probe = - 20C protect from light
Enzyme mix = -20C
Hemin substrate = 4C
Hemin standard = -20C
Hemin assay buffer = -20C
DMSO = -20C Read More

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I am sorryfor the confusion about the kit and for theinconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xx. It should be shipped out today. To check the st...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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Thank you for contacting us. Please find attached the COC requested. I haven't included the batch number you have received; if you want the batch number to be included let me know the batch number of kit received I will then send you an updated cer...

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Thank you for contacting us. We unfortunately do not provide certificate of analysis for our products we however can happily provide certificate of compliance. Please let us know if you are willing to receive Certificate of Compliance. I ...

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Thank you for your enquiry. I have contacted the originator of the product and they have confirmed that the Hemin Assay kit can measure both free and bound hemin. Regarding measurement, please refer to the datasheet for protocols on using this kit. ...

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