• Product name
    Hemin Assay Kit
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
  • Assay time
    0h 30m
  • Product overview

    Hemin Assay Kit (ab65332) utilizes peroxidase activity in the presence of hemin to provide a simple, exquisitely sensitive assay which causes the conversion of a colorless probe to a strongly colored (? = 570) compound. Trace amounts of Hemin can be quantitated in the 5-160 pg (10-250 fmol) range.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Free hemin results from the breakdown of hemin-containing proteins such as hemoglobin and myoglobin. It can be detected in various body fluids such as saliva, urine and csf under various pathological states. Free hemin exists in cells at a very minute concentrations (< 1µM ˜ 650 ng/ml) exerting regulatory functions such as repression of nonspecific d-aminolevulinate synthase expression and induction of microsomal hemin oxygenase-1. Hemin can stimulate growth of oral bacteria involved in gingivitis and is an indicator of possible pathological conditions when found in the urine or feces.

  • Tested applications
    Suitable for: Functional Studiesmore details


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 100 tests
    Enzyme Mix Blue 1 vial
    Hemin Assay Buffer WM 1 x 25ml
    Hemin Probe (in DMSO) Red 1 x 0.2ml
    Hemin Standard (1 nM) (Lyophilised) Yellow 1 vial
    Hemin Substrate Green 1 x 1ml
  • Research areas
  • Relevance
    Free hemin results from the breakdown of hemin-containing proteins such as hemoglobin and myoglobin. Hemin level has been used as an indicator of possible pathological conditions when found in the urine or faeces. It plays regulatory functions for enzyme activities.


Our Abpromise guarantee covers the use of ab65332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.


  • Uraskai et al investigates the effect of perturbations to uridine homeostasis on heme-deficiency response pathway. Mouse liver hemin concentration was measured using ab65332. 

  • Hemin measured in various biofluids showing concentration (nM)

  • A: Time course of development of color and B: Standard Curve at 30 minutes for Hemin standard.



This product has been referenced in:
  • Moreno-Navarrete JM  et al. Increased adipose tissue heme levels and exportation are associated with altered systemic glucose metabolism. Sci Rep 7:5305 (2017). Read more (PubMed: 28706239) »
  • Urasaki Y  et al. Uridine affects liver protein glycosylation, insulin signaling, and heme biosynthesis. PLoS One 9:e99728 (2014). Functional Studies . Read more (PubMed: 24918436) »

See all 2 Publications for this product

Customer reviews and Q&As

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The component should be stored as follows;

Probe = - 20C protect from light
Enzyme mix = -20C
Hemin substrate = 4C
Hemin standard = -20C
Hemin assay buffer = -20C
DMSO = -20C Read More

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I am sorryfor the confusion about the kit and for theinconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xx. It should be shipped out today. To check the st...

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I have unfortunately not yet heard back from the laboratory. I just sent a reminder now in order to get their opinion on the source of the problem.

In regards to the standa...

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I would like also to confirm, that the product is covered by our Abpromise guarantee in case we could not find a solution to the pos...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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Thank you for contacting us. Please find attached the COC requested. I haven't included the batch number you have received; if you want the batch number to be included let me know the batch number of kit received I will then send you an updated cer...

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Thank you for contacting us. We unfortunately do not provide certificate of analysis for our products we however can happily provide certificate of compliance. Please let us know if you are willing to receive Certificate of Compliance. I ...

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Thank you for your enquiry. I have contacted the originator of the product and they have confirmed that the Hemin Assay kit can measure both free and bound hemin. Regarding measurement, please refer to the datasheet for protocols on using this kit. ...

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