Overview

  • Product nameAnti-Hemogen antibody
    See all Hemogen primary antibodies
  • Description
    Rabbit polyclonal to Hemogen
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Hemogen.

    (Peptide available as ab66549.)

  • Positive control
    • This antibody gave a positive signal in the following Human Lysates: Testis Tissue, Ovary Tissue, Pancreas Tissue, Lymph node Tissue, Brain Tissue (data not shown), Spleen Tissue (data not shown), Thymus Tissue (data not shown), HeLa Whole Cell (data not shown), SW480 Whole Cell (data not shown), HepG2 Whole Cell (data not shown)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 55 kDa).

Target

  • FunctionRegulates the proliferation and differentiation of hematopoietic cells. Overexpression block the TPA-induced megakaryocytic differentiation in the K562 cell model. May also prevent cell apoptosis through the activation of the nuclear factor-kappa B (NF-kB).
  • Tissue specificityExpressed in hematopoietic precursor cells (at protein level). Detected in CD34+ and K562 leukemia cell line (at protein level). Expressed in bone marrow, testis, thymus. Also expressed in thymus and thyroid tumors, non-Hodgkin lymphoma, various leukemia cell lines, peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) of patients with leukemia.
  • Developmental stageExpressed in fetal liver, kidney and brain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EDAG antibody
    • EDAG-1 antibody
    • Erythroid differentiation-associated gene protein antibody
    • Hemgn antibody
    • HEMGN_HUMAN antibody
    • Hemogen antibody
    • Hemopoietic gene protein antibody
    • Negative differentiation regulator protein antibody
    see all

Anti-Hemogen antibody images

  • All lanes : Anti-Hemogen antibody (ab66550) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human ovary tissue lysate - total protein (ab30222)
    Lane 3 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 4 : Human lymph node tissue lysate - total protein (ab29871)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Hemogen antibody (ab66550)

ab66550 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"