Purification notesPurity was determined by SDS PAGE (blue stain), showing that the IgY(delta Fc) constitute was 90% of total protein, and the IgY and IgY (delta Fc) constitute was 95% of total protein.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/5000.
Immunochromatography: Use at an assay dependent dilution.
Immunoturbidimetry: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 15 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in oxygen transport from the lung to the various peripheral tissues. LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure. Spinorphin: functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.
Tissue specificityRed blood cells.
Involvement in diseaseHeinz body anemias Beta-thalassemia Sickle cell anemia Beta-thalassemia, dominant, inclusion body type
Sequence similaritiesBelongs to the globin family.
Post-translational modificationsGlucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus. S-nitrosylated; a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2). Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure. PubMed:16916647 reports the identification of HBB acetylated on Lys-145 in the cytosolic fraction of HeLa cells. This may have resulted from contamination of the sample.