• Product nameAnti-Hemoglobin A1c antibody
    See all Hemoglobin A1c primary antibodies
  • Description
    Rabbit polyclonal to Hemoglobin A1c
  • Specificityab31152 is specific to Hemoglobin A1c.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Hemoglobin A1c.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.14M Sodium chloride, 10mM PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity purified IgG is obtained by synthetic peptide coupling affinity chromatography. Purity was determined by SDS PAGE (blue stain), showing that the IgG constitute was 90% of total protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab31152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/2000.
    Immunochromatography: Use at an assay dependent dilution.
    Immunoturbidimetry: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 15 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in oxygen transport from the lung to the various peripheral tissues.
      LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure.
      Spinorphin: functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.
    • Tissue specificityRed blood cells.
    • Involvement in diseaseHeinz body anemias
      Sickle cell anemia
      Beta-thalassemia, dominant, inclusion body type
    • Sequence similaritiesBelongs to the globin family.
    • Post-translational
      Glucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus.
      S-nitrosylated; a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2).
      Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure. PubMed:16916647 reports the identification of HBB acetylated on Lys-145 in the cytosolic fraction of HeLa cells. This may have resulted from contamination of the sample.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beta-globin antibody
      • HBB antibody
      • HBB_HUMAN antibody
      • Hemoglobin beta chain antibody
      • Spinorphin antibody
      see all

    Anti-Hemoglobin A1c antibody images

    • All lanes : Anti-Hemoglobin A1c antibody (ab31152) at 1 µg/ml

      Lane 1 : Human thymus tissue lysate - total protein (ab30146)
      Lane 2 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 10 µg per lane.

      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 15 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)

    References for Anti-Hemoglobin A1c antibody (ab31152)

    This product has been referenced in:
    • Chen SP  et al. Gold nanoparticles-coated magnetic microspheres as affinity matrix for detection of hemoglobin A1c in blood by microfluidic immunoassay. Biosens Bioelectron 26:4779-84 (2011). Immunoelectrophoresis ; Human . Read more (PubMed: 21719268) »

    See 1 Publication for this product

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    We have not used ab31152 and ab82273 product together in ELISA. However based on the nature of protein and immunogen of antibody it seems that both of these product can work together. I am sorry we will not be abl...

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    Hemoglobin A1c is formed by the non-enzymatic attachment of glucose to the N-terminal valine of the β-chain of hemoglobin. ab31152 antibody is specifically designed to detect hemoglobin A1c. Hemoblobin is a heterodimer composed of an alpha and beta...

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