- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-Hemoglobin antibody
See all Hemoglobin primary antibodies -
DescriptionGoat polyclonal to Hemoglobin
-
Tested applicationsSuitable for: Immunoelectrophoresis, Double Immunodiffusionmore details
-
Species reactivityReacts with: Human
-
Immunogen
Human Hemoglobin F.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 2mg/ml Sodium chloride, 100mM Tris. pH 7.2 -
Concentration information loading... -
PurityIgG fraction
-
Purification notesAntiserum was solid phase adsorbed against hemoglobin A to ensure specificity. The antiserum was fractionated and passed over DEAE to yield an IgG fraction.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab19363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunoelectrophoresis | Use at an assay dependent dilution. | |
| Double Immunodiffusion | Use at an assay dependent dilution. |
Target
-
FunctionInvolved in oxygen transport from the lung to the various peripheral tissues.
-
Tissue specificityRed blood cells.
-
Involvement in diseaseDefects in HBA1/HBA2 may be a cause of Heinz body anemias (HEIBAN) [MIM:140700]. This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency.
Defects in HBA1/HBA2 are the cause of alpha-thalassemia (A-THAL) [MIM:604131]. The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non-deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers.
Note=Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders. -
Sequence similaritiesBelongs to the globin family.
-
Post-translational
modificationsThe initiator Met is not cleaved in variant Thionville and is acetylated. - Information by UniProt
-
Database links
- Entrez Gene: 3039 Human
- Entrez Gene: 3040 Human
- Entrez Gene: 3043 Human
- Entrez Gene: 3047 Human
- Omim: 141800 Human
- Omim: 141900 Human
- Omim: 142200 Human
- SwissProt: P68871 Human
see all -
Alternative names
- 3-prime alpha-globin gene antibody
- Alpha 2 globin chain antibody
- Alpha globin antibody
see all
References for Anti-Hemoglobin antibody (ab19363)
ab19363 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"