Anti-Hemoglobin subunit alpha antibody (ab102758)

Overview

  • Product name
    Anti-Hemoglobin subunit alpha antibody
    See all Hemoglobin subunit alpha primary antibodies
  • Description
    Rabbit polyclonal to Hemoglobin subunit alpha
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    KLH conjugated synthetic peptide corresponding to a region within internal sequence amino acids 107-136 of Human Hemoglobin subunit alpha.

  • Positive control
    • Mouse liver tissue lysate; Human lung carcinoma tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab102758 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab102758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 15 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Involved in oxygen transport from the lung to the various peripheral tissues.
  • Tissue specificity
    Red blood cells.
  • Involvement in disease
    Heinz body anemias
    Alpha-thalassemia
    Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
    Hemoglobin H disease
  • Sequence similarities
    Belongs to the globin family.
  • Post-translational
    modifications
    The initiator Met is not cleaved in variant Thionville and is acetylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 globin antibody
    • Alpha globin antibody
    • Alpha one globin antibody
    • Alpha-globin antibody
    • HBA_HUMAN antibody
    • HBA1 antibody
    • HBA2 antibody
    • Hemoglobin alpha 1 antibody
    • Hemoglobin alpha 1 chain antibody
    • Hemoglobin alpha 1 globin chain antibody
    • Hemoglobin alpha 2 antibody
    • Hemoglobin alpha chain antibody
    • Hemoglobin subunit alpha antibody
    • MGC126895 antibody
    • MGC126897 antibody
    see all

Images

  • Anti-Hemoglobin subunit alpha antibody (ab102758) at 1/100 dilution + Mouse liver tissue lysate at 35 µg

    Predicted band size : 15 kDa
  • ab102758, at 1/50 dilution, staining Hemoglobin subunit alpha in formalin-fixed, paraffin-embedded Human lung carcinoma using a peroxidase-conjugated secondary antibody and DAB staining.

References

This product has been referenced in:
  • Htun NM  et al. Near-infrared autofluorescence induced by intraplaque hemorrhage and heme degradation as marker for high-risk atherosclerotic plaques. Nat Commun 8:75 (2017). Read more (PubMed: 28706202) »
  • Shephard F  et al. Analysis of Mitochondrial haemoglobin in Parkinson's disease brain. Mitochondrion 29:45-52 (2016). WB . Read more (PubMed: 27181046) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you very much for your call today and for letting us know about the trouble with this new vial of ab102758.

As we discussed, I'm sending a free of charge replacement vial of this antibody on the order ***, which should arrive on Monday....

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up