Anti-Hemoglobin subunit alpha antibody (ab82871)

Overview

  • Product nameAnti-Hemoglobin subunit alpha antibody
    See all Hemoglobin subunit alpha primary antibodies
  • Description
    Rabbit polyclonal to Hemoglobin subunit alpha
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Chimpanzee, Monkey, Baboon, Macaque Monkey, Rhesus monkey, Gorilla, Dolphin, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Hemoglobin subunit alpha aa 100 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab93083, ab93083)

  • Positive control
    • This antibody gave a positive signal in human bone marrow and spleen tissue lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/250. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).

Target

  • FunctionInvolved in oxygen transport from the lung to the various peripheral tissues.
  • Tissue specificityRed blood cells.
  • Involvement in diseaseHeinz body anemias
    Alpha-thalassemia
    Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
    Hemoglobin H disease
  • Sequence similaritiesBelongs to the globin family.
  • Post-translational
    modifications
    The initiator Met is not cleaved in variant Thionville and is acetylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 globin antibody
    • Alpha globin antibody
    • Alpha one globin antibody
    • Alpha-globin antibody
    • HBA_HUMAN antibody
    • HBA1 antibody
    • HBA2 antibody
    • Hemoglobin alpha 1 antibody
    • Hemoglobin alpha 1 chain antibody
    • Hemoglobin alpha 1 globin chain antibody
    • Hemoglobin alpha 2 antibody
    • Hemoglobin alpha chain antibody
    • Hemoglobin subunit alpha antibody
    • MGC126895 antibody
    • MGC126897 antibody
    see all

Anti-Hemoglobin subunit alpha antibody images

  • All lanes : Anti-Hemoglobin subunit alpha antibody (ab82871) at 1/250 dilution

    Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • IHC image of Hemoglobin A1c staining in human bone marrow formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab82871, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab17976 staining Hemoglobin A1c in Rat Lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA in PBS for 20 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hours at 25°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody

    See Abreview

References for Anti-Hemoglobin subunit alpha antibody (ab82871)

ab82871 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (lung)
Specification lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Sep 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"