Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444)


  • Product nameAnti-Hemoglobin subunit alpha antibody [EP3607]
    See all Hemoglobin subunit alpha primary antibodies
  • Description
    Rabbit monoclonal [EP3607] to Hemoglobin subunit alpha
  • SpecificityMouse and rat tissues tested (lung and kidney) gave negative or low sensitivity staining in IHC-P. This antibody may not be suitable for IHC with mouse or rat samples. If you have any questions, please contact our Scientific Support team.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Hemoglobin subunit alpha.

  • Positive control
    • WB: K562,fetal liver, and fetal heart lysates IHC-P: Human spleen and Human colon tissues
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP3607
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab109444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • FunctionInvolved in oxygen transport from the lung to the various peripheral tissues.
    • Tissue specificityRed blood cells.
    • Involvement in diseaseHeinz body anemias
      Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
      Hemoglobin H disease
    • Sequence similaritiesBelongs to the globin family.
    • Post-translational
      The initiator Met is not cleaved in variant Thionville and is acetylated.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha 1 globin antibody
      • Alpha globin antibody
      • Alpha one globin antibody
      • Alpha-globin antibody
      • HBA_HUMAN antibody
      • HBA1 antibody
      • HBA2 antibody
      • Hemoglobin alpha 1 antibody
      • Hemoglobin alpha 1 chain antibody
      • Hemoglobin alpha 1 globin chain antibody
      • Hemoglobin alpha 2 antibody
      • Hemoglobin alpha chain antibody
      • Hemoglobin subunit alpha antibody
      • MGC126895 antibody
      • MGC126897 antibody
      see all

    Anti-Hemoglobin subunit alpha antibody [EP3607] images

    • All lanes : Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444) at 1/1000 dilution

      Lane 1 : K562 lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : Fetal heart lysates

      Lysates/proteins at 10 µg per lane.

      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 15 kDa
      Observed band size : 14 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of Hemoglobin subunit alpha in paraffin embedded Human spleen tissue, using ab109444 at a 1/250 dilution.
    • Immunohistochemical analysis of Hemoglobin subunit alpha in paraffin embedded Human colon tissue, using ab109444 at a 1/250 dilution.

    References for Anti-Hemoglobin subunit alpha antibody [EP3607] (ab109444)

    ab109444 has not yet been referenced specifically in any publications.

    Product Wall

    Vielen Dank für Ihren Anruf.

    Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456und so...

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Thank you for your reply and for testing the antibodies.

    Unfortunately, we do not have another lot in stock for ab109444 - just the same lot. Please let me know if one of the other Anti-Hemoglobin subunit alpha antibodies we carry, ab92492 o...

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    According to our records, ab6721 or ab109444 was proving difficult to use in WBand we were in contact in order to help resolve the issue.

    Looking at our correspondence, it appears that we are awaiting more details in order to help us better ...

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    Thank you for contacting us.
    I am sorry to hear that one of these antibodies is notworking properly.

    As we discussed over the phone, trying a no-primary control (with secondary antibody only) would be another way to check if the problem...

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    Thank you for the reply.

    The recommended dilutions on our datasheets provide a concentration to begin with, however the optimal dilution does have to be determined by the end user. Therefore I strongly recommend that you try various concentra...

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    Thank you for sending that protocol information.

    If I have read the information correctly, then there are a few protocol variations that may work:

    1 - Did the customer only try a 1/5000 primary antibody dilution? If so, I strongly s...

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    I am sorry that the replacement antibody did not prove to succesful. Unfortunately the protocol that you attached did not come through. Could you resend the protocol so that I can look it over and see if there are any suggestions that I can make.

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