Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)


  • Product nameAnti-Hemoglobin subunit alpha antibody [EPR3608]
    See all Hemoglobin subunit alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR3608] to Hemoglobin subunit alpha
  • SpecificityThis antibody gave a positive signal in Western blotting of rat brain, heart, kidney and spleen tissue. Mouse and rat tissues (including lung, spleen, and kidney) gave negative staining in IHC-P. This antibody may not be suitable for IHC with mouse or rat samples. If you have any questions, please contact our Scientific Support team.
  • Tested applicationsSuitable for: ELISA, WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to amino acids between 80 and 105 in Human Hemoglobin (alpha chain).

  • Positive control
    • K562 cell lysate, human heart lysate, human spleen tissue, human stomach cancer tissue, human lung tissue, human placenta
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab92492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).Can be blocked with Hemoglobin subunit alpha peptide (ab206105).
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

This antibody may not be suitable for IHC of mouse or rat tissues.

Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • FunctionInvolved in oxygen transport from the lung to the various peripheral tissues.
  • Tissue specificityRed blood cells.
  • Involvement in diseaseHeinz body anemias
    Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
    Hemoglobin H disease
  • Sequence similaritiesBelongs to the globin family.
  • Post-translational
    The initiator Met is not cleaved in variant Thionville and is acetylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 1 globin antibody
    • Alpha globin antibody
    • Alpha one globin antibody
    • Alpha-globin antibody
    • HBA_HUMAN antibody
    • HBA1 antibody
    • HBA2 antibody
    • Hemoglobin alpha 1 antibody
    • Hemoglobin alpha 1 chain antibody
    • Hemoglobin alpha 1 globin chain antibody
    • Hemoglobin alpha 2 antibody
    • Hemoglobin alpha chain antibody
    • Hemoglobin subunit alpha antibody
    • MGC126895 antibody
    • MGC126897 antibody
    see all

Anti-Hemoglobin subunit alpha antibody [EPR3608] images

  • All lanes : Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Human heart lysate

    Lysates/proteins at 10 µg per lane.

    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 15 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
  • ab92492 at 1/100 dilution staining hemoglobin in human spleen tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

  • Immunohistochemical analysis of paraffin embedded human placenta tissue labeling hemoglobin subunit alpha with ab92492 at 0.051 µg/ml (1:40000 dilution).

  • Immunohistochemical analysis of paraffin embedded human stomach cancer tissue labeling hemoglobin subunit alpha with ab92492 at 0.051 µg/ml (1:40000 dilution).

  • Immunohistochemical analysis of paraffin embedded human placenta tissue labeling hemoglobin subunit alpha with ab92492 at 0.051 µg/ml (1:40000 dilution).

References for Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

This product has been referenced in:
  • Banerjee S  et al. Haptoglobin alters oxygenation and oxidation of hemoglobin and decreases propagation of peroxide-induced oxidative reactions. Free Radic Biol Med 53:1317-26 (2012). WB . Read more (PubMed: 22841869) »

See 1 Publication for this product

Product Wall

I have processed your request to receive ab109444 as a replacement for ab92492. I have also requested that you receive a new vial of secondary antibody and a new HRP substrate kit as well. I do not have an estimated time of arrival yet, but I will keep...

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Vielen Dank für Ihren Anruf.

Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456und so...

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Thank you for contacting us. I am sorry to hear that ab92492 is producing unsatisfactory results in WB. All of our Hemoglobin subunit alpha antibodies have been validated for use on endogenous proteins, but have not been tested on purified hemoglobin p...

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Thank you for sending that protocol information.

If I have read the information correctly, then there are a few protocol variations that may work:

1 - Did the customer only try a 1/5000 primary antibody dilution? If so, I strongly s...

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Thank you for your interest in Abcam. We continually add antibodies and other protein detection products to our catalogue but we do not offer custom-made antibodies. However, we are happy to pass on the contact details of the following companies th...

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I have talked to my colleagues and I will be able to send a new secondary antibody aswell as a new ECL kit with the replacement antibody. I will send the new products as soon as*****decides which antibody he would like to receive, either ab109444 or a...

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Gracias por contactarnos de nuevo y enviarnos más información acerca del protocolo seguido. -¿Habéis llevado a cabo un control no primario? Esto es, incubar directamente el secundario y observar si existe tinción sin necesidad de haber añadido el pri...

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Application Immunocytochemistry
Sample Mouse Cultured Cells (Erythroleukemia cell line)
Specification Erythroleukemia cell line
Fixative None
Permeabilization No

Abcam user community

Verified customer

Submitted Apr 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Recombinant protein (Recombinant HBA1)
Specification Recombinant HBA1
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Type Indirect

Abcam user community

Verified customer

Submitted Mar 07 2011