Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

Overview

  • Product nameAnti-Hemoglobin subunit alpha antibody [EPR3608]
    See all Hemoglobin subunit alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR3608] to Hemoglobin subunit alpha
  • SpecificityThis antibody gave a positive signal in Western blotting of rat brain, heart, kidney and spleen tissue. This antibody works in human, mouse and rat in IHC.
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Hemoglobin subunit alpha aa 50-150.
    (Peptide available as ab206105)

  • Positive control
    • K562 cell lysate, human heart lysate, human spleen tissue, human stomach cancer tissue, human lung tissue, human placenta, rat heart lysate, mouse spleen lysate and mouse cerebral cortex.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab92492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Detects a band of approximately 14 kDa (predicted molecular weight: 15 kDa).Can be blocked with Hemoglobin subunit alpha peptide (ab206105).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol)

Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionInvolved in oxygen transport from the lung to the various peripheral tissues.
    • Tissue specificityRed blood cells.
    • Involvement in diseaseHeinz body anemias
      Alpha-thalassemia
      Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
      Hemoglobin H disease
    • Sequence similaritiesBelongs to the globin family.
    • Post-translational
      modifications
      The initiator Met is not cleaved in variant Thionville and is acetylated.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha 1 globin antibody
      • Alpha globin antibody
      • Alpha one globin antibody
      • Alpha-globin antibody
      • HBA_HUMAN antibody
      • HBA1 antibody
      • HBA2 antibody
      • Hemoglobin alpha 1 antibody
      • Hemoglobin alpha 1 chain antibody
      • Hemoglobin alpha 1 globin chain antibody
      • Hemoglobin alpha 2 antibody
      • Hemoglobin alpha chain antibody
      • Hemoglobin subunit alpha antibody
      • MGC126895 antibody
      • MGC126897 antibody
      see all

    Anti-Hemoglobin subunit alpha antibody [EPR3608] images

    • All lanes : Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492) at 1/2000 dilution (purified)

      Lane 1 : Human heart lysate
      Lane 2 : Rat heart lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size : 15 kDa
      Observed band size : 14 kDa (why is the actual band size different from the predicted?)

      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492) at 1/10000 dilution (purified) + Mouse spleen lysate at 15 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size : 15 kDa
      Observed band size : 14 kDa (why is the actual band size different from the predicted?)

      Blocking and diluting buffer and concentration: 5% NFDM /TBST. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue labeling Hemoglobin subunit alpha antibody with purified ab92492 at 1/500 dilution (1.4 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. ab97051, a HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody at 1/500 dilution. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • All lanes : Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492) at 1/1000 dilution (unpurified)

      Lane 1 : K562 cell lysate
      Lane 2 : Human heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 15 kDa
      Observed band size : 14 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin embedded human placenta tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 µg/ml (1:40000 dilution).

    • Immunohistochemical analysis of paraffin embedded human stomach cancer tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 µg/ml (1:40000 dilution).

    • Immunohistochemical analysis of paraffin embedded human placenta tissue labeling hemoglobin subunit alpha with unpurified ab92492 at 0.051 µg/ml (1:40000 dilution).

    References for Anti-Hemoglobin subunit alpha antibody [EPR3608] (ab92492)

    This product has been referenced in:
    • Wang Y  et al. Role of hemoglobin and transferrin in multi-wall carbon nanotube-induced mesothelial injury and carcinogenesis. Cancer Sci 107:250-7 (2016). Rat . Read more (PubMed: 26679080) »
    • Banerjee S  et al. Haptoglobin alters oxygenation and oxidation of hemoglobin and decreases propagation of peroxide-induced oxidative reactions. Free Radic Biol Med 53:1317-26 (2012). WB . Read more (PubMed: 22841869) »

    See all 2 Publications for this product

    Product Wall

    I have processed your request to receive ab109444 as a replacement for ab92492. I have also requested that you receive a new vial of secondary antibody and a new HRP substrate kit as well. I do not have an estimated time of arrival yet, but I will keep...

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    Vielen Dank für Ihren Anruf.

    Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456und so...

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    Thank you for contacting us. I am sorry to hear that ab92492 is producing unsatisfactory results in WB. All of our Hemoglobin subunit alpha antibodies have been validated for use on endogenous proteins, but have not been tested on purified hemoglobin p...

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    Thank you for sending that protocol information.

    If I have read the information correctly, then there are a few protocol variations that may work:

    1 - Did the customer only try a 1/5000 primary antibody dilution? If so, I strongly s...

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    Thank you for your interest in Abcam. We continually add antibodies and other protein detection products to our catalogue but we do not offer custom-made antibodies. However, we are happy to pass on the contact details of the following companies th...

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    I have talked to my colleagues and I will be able to send a new secondary antibody aswell as a new ECL kit with the replacement antibody. I will send the new products as soon as*****decides which antibody he would like to receive, either ab109444 or a...

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    Gracias por contactarnos de nuevo y enviarnos más información acerca del protocolo seguido. -¿Habéis llevado a cabo un control no primario? Esto es, incubar directamente el secundario y observar si existe tinción sin necesidad de haber añadido el pri...

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    Application Immunocytochemistry
    Sample Mouse Cultured Cells (Erythroleukemia cell line)
    Specification Erythroleukemia cell line
    Fixative None
    Permeabilization No
    Username

    Abcam user community

    Verified customer

    Submitted Apr 21 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application ELISA
    Sample Human Recombinant protein (Recombinant HBA1)
    Specification Recombinant HBA1
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Type Indirect
    Username

    Abcam user community

    Verified customer

    Submitted Mar 07 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"