Recombinant Anti-Hemoglobin subunit alpha antibody [SP212] (ab227671)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP212] to Hemoglobin subunit alpha
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Hemoglobin subunit alpha antibody [SP212]
See all Hemoglobin subunit alpha primary antibodies -
Description
Rabbit monoclonal [SP212] to Hemoglobin subunit alpha -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human heart tissue lysate. IHC-P: Human placenta, spleen and skeletal muscle tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP212 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
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WB |
1/400. Predicted molecular weight: 15 kDa.
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
WB
1/400. Predicted molecular weight: 15 kDa. |
Target
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Function
Involved in oxygen transport from the lung to the various peripheral tissues. -
Tissue specificity
Red blood cells. -
Involvement in disease
Heinz body anemias
Alpha-thalassemia
Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
Hemoglobin H disease -
Sequence similarities
Belongs to the globin family. -
Post-translational
modificationsThe initiator Met is not cleaved in variant Thionville and is acetylated. - Information by UniProt
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Database links
- Entrez Gene: 3039 Human
- Entrez Gene: 3040 Human
- Omim: 141800 Human
- Omim: 141850 Human
- SwissProt: P69905 Human
- Unigene: 449630 Human
- Unigene: 654744 Human
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Alternative names
- Alpha 1 globin antibody
- Alpha globin antibody
- Alpha one globin antibody
see all
Images
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Formalin-fixed paraffin-embedded human spleen tissue stained for Hemoglobin subunit alpha with ab227671 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed paraffin-embedded human placenta tissue stained for Hemoglobin subunit alpha with ab227671 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed paraffin-embedded human skeletal muscle tissue stained for Hemoglobin subunit alpha with ab227671 at 1/100 dilution in immunohistochemical analysis.
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Anti-Hemoglobin subunit alpha antibody [SP212] (ab227671) at 1/400 dilution + Human heart tissue lysate.
Developed using the ECL technique.
Predicted band size: 15 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227671 has not yet been referenced specifically in any publications.