Overview

  • Product nameAnti-Hemopexin antibody
    See all Hemopexin primary antibodies
  • Description
    Goat polyclonal to Hemopexin
  • Tested applicationsSuitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from Human Hemopexin.

  • Positive control
    • Human liver and placenta (serum) tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab110126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 3.75 µg/ml. ab110126 was validated for use in Immunohistochemistry on a panel of 21 formalin fixed, paraffin Human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated Goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Target

  • FunctionBinds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Sequence similaritiesBelongs to the hemopexin family.
    Contains 5 hemopexin-like domains.
  • Post-translational
    modifications
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta 1B glycoprotein antibody
    • Beta-1B-glycoprotein antibody
    • FLJ56652 antibody
    • HEMO antibody
    • HEMO_HUMAN antibody
    • Hemopexin antibody
    • Hpx antibody
    • HX antibody
    see all

Anti-Hemopexin antibody images

  • Immunohistochemical analysis of Hemopexin expression in formalin fixed, paraffin embedded Human liver, using ab110126 at 3.75 µg/ml.
  • Immunohistochemical analysis of Hemopexin expression in formalin fixed, paraffin embedded Human placenta, serum, using ab110126 at 3.75 µg/ml.

References for Anti-Hemopexin antibody (ab110126)

ab110126 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Mouse Tissue sections (liver)
Specification liver
Permeabilization No
Fixative Methanol
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Abcam user community

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Submitted Oct 01 2014

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
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Submitted Dec 26 2012

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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Submitted Jul 20 2012

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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Abcam user community

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Submitted Jul 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 07 2011

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization No
Blocking step No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Shakil Ahmad

Verified customer

Submitted Aug 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"