The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 3.75 µg/ml. ab110126 was validated for use in Immunohistochemistry on a panel of 21 formalin fixed, paraffin Human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated Goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.
FunctionBinds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
Tissue specificityExpressed by the liver and secreted in plasma.
Sequence similaritiesBelongs to the hemopexin family. Contains 5 hemopexin-like domains.
Post-translational modificationsN- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.