Overview

  • Product name
  • Description
    Rabbit polyclonal to Hemopexin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Rabbit, Human, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Hemopexin.

  • Positive control
    • This antibody gave a positive signal in both Mouse and Rat Sciatic Nerve tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab116532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 52 kDa).

Target

Images

  • All lanes : Anti-Hemopexin antibody (ab116532) at 1 µg/ml

    Lane 1 : Mouse Sciatic Nerve Tissue Lysate
    Lane 2 : Rat Sciatic Nerve Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 70 kDa (why is the actual band size different from the predicted?)


    Exposure time: 1 minute


    Hemopexin has been shown to migrate at ~70 kDa under reducing conditions by Madore et al PMID: 9930744. We welcome customer feedback. Hemopexin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab116532 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab116532 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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