Overview

  • Product nameAnti-Hemopexin antibody
    See all Hemopexin primary antibodies
  • Description
    Rabbit polyclonal to Hemopexin
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the Center region of Human Hemopexin.

  • Positive control
    • Mouse liver lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab90947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 52 kDa.
ELISA 1/1000.

Target

  • FunctionBinds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Sequence similaritiesBelongs to the hemopexin family.
    Contains 5 hemopexin-like domains.
  • Post-translational
    modifications
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta 1B glycoprotein antibody
    • Beta-1B-glycoprotein antibody
    • FLJ56652 antibody
    • HEMO antibody
    • HEMO_HUMAN antibody
    • Hemopexin antibody
    • Hpx antibody
    • HX antibody
    see all

Anti-Hemopexin antibody images

  • Anti-Hemopexin antibody (ab90947) at 1/1000 dilution + Mouse liver tissue lysate at 35 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 52 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST.

References for Anti-Hemopexin antibody (ab90947)

This product has been referenced in:
  • Nath KA  et al. Age sensitizes the kidney to heme protein-induced acute kidney injury. Am J Physiol Renal Physiol 304:F317-25 (2013). Mouse . Read more (PubMed: 23195679) »
  • Epple LM  et al. Medulloblastoma exosome proteomics yield functional roles for extracellular vesicles. PLoS One 7:e42064 (2012). WB . Read more (PubMed: 22848702) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse Kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9
Permeabilization Yes - 0.1% TRITON
Specification Mouse Kidney
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 2%
Fixative Paraformaldehyde
Username

Dra. Marta Garcia Ramirez

Verified customer

Submitted Jan 22 2016

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HepG2)
Specification HepG2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 01 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 01 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Sample Human Tissue sections (human liver)
Specification human liver
Permeabilization Yes - 0.2% Triton X-100 and PBS
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 13 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jul 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jul 08 2013


I would suggest running the gel under reducing conditions as the protein has 6 disulfide bonds which can change the protein's running behaviour. This will linearize the protein.

Also the protein has multiple glycosylation sites which will...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Dec 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2)
Loading amount 50 µg
Specification HepG2
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Dec 25 2012

Application Western blot
Sample Rat Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 01 2011

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