Hemopexin (HPX) Human ELISA Kit (ab108859)

Overview

  • Product nameHemopexin (HPX) Human ELISA KitSee all Hemopexin kits ...
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    > 0.7 µg/ml
  • Range
    0.078 µg/ml - 5 µg/ml
  • Recovery

    98 %

  • Assay time
    2h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Hemopexin (HPX) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of hemopexin levels in plasma and serum.

    A Hemopexin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Hemopexin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Hemopexin captured in plate.

  • Tested applicationsCompetitive ELISA more details
  • PlatformMicroplate

Properties

  • RelevanceBinds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
  • Cellular localizationSecreted
  • Alternative names
    • beta-1B-glycoprotein
    • HPX
    • Hpxn
    • HX
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Competitive ELISA Use at an assay dependent concentration.

Hemopexin (HPX) Human ELISA Kit images

  • Representative Standard Curve using ab108859.

Protocols

References for Hemopexin (HPX) Human ELISA Kit (ab108859)

ab108859 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit
2)...

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Thank you for your enquiry.

Sorry for the confusion here. A large excess of Standard is provided in this ELISA kit. To properly dilute the standard, just reconstitute the 3.5ug Standard you received in 0.7ml of Diluent to produce a 5ug/ml Sta...

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Abreview for Hemopexin Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Serum
Amount of Sample: 2 ul µg
Type of Kit Used: ELISA
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: We diluted 2ul of plasma with 800 ul of Diluent (1:400).
We washed the plate 6 times with a machine.
The standards data generated an excelent curve with a fitting error of
3.48e-5.
All samples assayed showed data inside the curve.
Username

Dr. Rafael Montes Nieto

Verified customer

Submitted Mar 26 2012

Thank you for your phone call. Please find testing data for ab108859 attached. I hope this helps, please let me know if you need any additional information or assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"