Anti-HENMT1 antibody (ab121991)
Key features and details
- Rabbit polyclonal to HENMT1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HENMT1 antibody -
Description
Rabbit polyclonal to HENMT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: MEENNLQCSS VVDGNFEEVP RETAIQFKPP LYRQRYQFVK NLVDQHEPKK VADLGCGDTS LLRLLKVNPC IELLVGV, corresponding to N terminal amino acids 1-77 of Human HENMT1.
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Positive control
- WB: HENMT1 overexpression HEK293 cell lysate. IHC-P: Human testis, colon and lymph node tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis. -
Sequence similarities
Belongs to the methyltransferase superfamily. HEN1 family. -
Cellular localization
Cytoplasm. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. - Information by UniProt
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Database links
- Entrez Gene: 113802 Human
- Omim: 612178 Human
- SwissProt: Q5T8I9 Human
- Unigene: 7962 Human
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Alternative names
- C1orf59 antibody
- HEN1 antibody
- HEN1 methyltransferase homolog 1 (Arabidopsis) antibody
see all
Images
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Immunohistochemical analysis of human testis tissue labeling HENMT1 with ab121991 at a 1/200 dilution.
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All lanes : Anti-HENMT1 antibody (ab121991) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells -
Immunohistochemical analysis of human colon tissue labeling HENMT1 with ab121991 at a 1/200 dilution.
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Immunohistochemical analysis of human lymph node tissue labeling HENMT1 with ab121991 at a 1/200 dilution.
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Immunohistochemical analysis of human pancreas tissue labeling HENMT1 with ab121991 at a 1/200 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121991 has not yet been referenced specifically in any publications.