Overview

  • Product name
    Anti-HENMT1 antibody
  • Description
    Rabbit polyclonal to HENMT1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: MEENNLQCSS VVDGNFEEVP RETAIQFKPP LYRQRYQFVK NLVDQHEPKK VADLGCGDTS LLRLLKVNPC IELLVGV, corresponding to N terminal amino acids 1-77 of Human HENMT1.

  • Positive control
    • HENMT1 overexpression HEK293 cell lysate; Human lymph node tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis.
  • Sequence similarities
    Belongs to the methyltransferase superfamily. HEN1 family.
  • Cellular localization
    Cytoplasm. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1orf59 antibody
    • HEN1 antibody
    • HEN1 methyltransferase homolog 1 (Arabidopsis) antibody
    • HEN1 methyltransferase homolog 1 antibody
    • HENMT_HUMAN antibody
    • Henmt1 antibody
    • RP11-256E16.2 antibody
    • Small RNA 2' O methyltransferase antibody
    • Small RNA 2''-O-methyltransferase antibody
    see all

Images

  • ab121991, at 1/75, staining HENMT1 in Paraffin-emebedded Human lymph node tissue by Immunohistochemistry.
  • All lanes : Anti-HENMT1 antibody (ab121991) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells

References

ab121991 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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