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High molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG. The antibody was raised against the total Heparan Sulphate Proteoglycan protein; no epitope mapping was ever performed.
Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.
Our Abpromise guarantee covers the use of ab2501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/25 - 1/200. Use amplification with ABC (avidin biotin complex).|
|IHC-P||Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Use amplification with ABC (avidin biotin complex).|
|IHC-Fr||Use at an assay dependent dilution. PubMed: 16055495Use amplification with ABC (avidin biotin complex).|
|IP||Use at an assay dependent dilution.|
|WB||1/100 - 1/1000.|
IHC image on a frozen section of human colon showing strong reactivity in the extracellular matrix and basement membrane.
ab2501 staining Heparan Sulphate Proteoglycan (Large) in human tonsil tissue sections by Immunohistochemistry (resin sections). Samples were incubated with primary antibody (1/100 in TBS) for 16 hours at 25°C. A biotin-conjugated rabbit anti-rat IgG polyclonal (1/500) was used as the secondary antibody.
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