Overview

  • Product nameAnti-Heparanase 1 antibody
    See all Heparanase 1 primary antibodies
  • Description
    Rabbit polyclonal to Heparanase 1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Monkey
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Heparanase 1.

    (Peptide available as ab45330.)

  • Positive control
    • WB: HeLa, Jurkat, A431, HEK293, HepG2, MCF-7, SHSY-5Y, and U20S (data not shown) whole cell lysates. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab42817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionEndoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Also implicated in the extravasation of leukocytes and tumor cell lines. Due to its contribution to metastasis and angiogenesis, it is considered to be a potential target for anti-cancer therapies.
  • Tissue specificityHighly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues.
  • Sequence similaritiesBelongs to the glycosyl hydrolase 79 family.
  • Post-translational
    modifications
    Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, 8 kDa and 50 kDa product. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme.
    N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility.
  • Cellular localizationLysosome membrane. Secreted. Secreted, internalised and transferred to late endosomes/lysosomes as a proheparanase. In lysosomes, it is processed into the active form, the heparanase. The uptake or internalisation of proheparanase is mediated by HSPGs. Heparin appears to be a competitor and retain proheparanase in the extracellular medium.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endo glucoronidase antibody
    • Endo-glucoronidase antibody
    • HEP antibody
    • Heparanase 50 kDa subunit antibody
    • Heparanase antibody
    • Heparanase-1 antibody
    • Heparanase1 antibody
    • Hpa 1 antibody
    • HPA antibody
    • Hpa1 antibody
    • HPR 1 antibody
    • HPR1 antibody
    • HPSE 1 antibody
    • HPSE antibody
    • HPSE_HUMAN antibody
    • HPSE1 antibody
    • HSE 1 antibody
    • HSE1 antibody
    see all

Anti-Heparanase 1 antibody images

  • ICC/IF image of ab stained HepG2 cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96899, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • All lanes : Anti-Heparanase 1 antibody (ab42817) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 52 kDa (possible cleavage fragment).

    Heparanase 1 is proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a 8 kDa and a 50 kDa product. We believe that the band observed at 52 kDa represents the 50 kDa product.

References for Anti-Heparanase 1 antibody (ab42817)

This product has been referenced in:
  • Hoedt E  et al. SILAC-based proteomic profiling of the human MDA-MB-231 metastatic breast cancer cell line in response to the two antitumoral lactoferrin isoforms: the secreted lactoferrin and the intracellular delta-lactoferrin. PLoS One 9:e104563 (2014). WB ; Human . Read more (PubMed: 25116916) »

See 1 Publication for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Monkey Cell lysate - whole cell (artery, liver, muscle)
Loading amount 40 µg
Specification artery, liver, muscle
Gel Running Conditions Reduced Denaturing (4-20% TG gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Oct 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"