Overview

  • Product nameAnti-Heparanase 1 antibody
    See all Heparanase 1 primary antibodies
  • Description
    Mouse monoclonal to Heparanase 1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: LILLGSPKLR TLARGLSPAY LRFGGTKTDF LIFDPKKEST FEERSYWQSQ VNQDICKYGS IPPDVEEKLR LEWPYQEQLL LREHYQKKFK NSTYSRSSV, corresponding to amino acids 72-171 of Human Heparanase 1

Properties

Applications

Our Abpromise guarantee covers the use of ab56781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEndoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Also implicated in the extravasation of leukocytes and tumor cell lines. Due to its contribution to metastasis and angiogenesis, it is considered to be a potential target for anti-cancer therapies.
    • Tissue specificityHighly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues.
    • Sequence similaritiesBelongs to the glycosyl hydrolase 79 family.
    • Post-translational
      modifications
      Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, 8 kDa and 50 kDa product. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme.
      N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility.
    • Cellular localizationLysosome membrane. Secreted. Secreted, internalised and transferred to late endosomes/lysosomes as a proheparanase. In lysosomes, it is processed into the active form, the heparanase. The uptake or internalisation of proheparanase is mediated by HSPGs. Heparin appears to be a competitor and retain proheparanase in the extracellular medium.
    • Information by UniProt
    • Database links
    • Alternative names
      • Endo glucoronidase antibody
      • Endo-glucoronidase antibody
      • HEP antibody
      • Heparanase 50 kDa subunit antibody
      • Heparanase antibody
      • Heparanase-1 antibody
      • Heparanase1 antibody
      • Hpa 1 antibody
      • HPA antibody
      • Hpa1 antibody
      • HPR 1 antibody
      • HPR1 antibody
      • HPSE 1 antibody
      • HPSE antibody
      • HPSE_HUMAN antibody
      • HPSE1 antibody
      • HSE 1 antibody
      • HSE1 antibody
      see all

    Anti-Heparanase 1 antibody images

    • Western blot against tagged recombinant protein immunogen using ab56781 Heparanase 1 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-Heparanase 1 antibody (ab56781)

    ab56781 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab56781.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"