Overview

  • Product name
    Anti-Heparanase 1 antibody
    See all Heparanase 1 primary antibodies
  • Description
    Rabbit polyclonal to Heparanase 1
  • Specificity
    This antibody reacts specifically with human 62 kDa HPSE protein, Heparanase 50 kDa subunit and Heparanase 8 kDa subunit.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide derived from N terminal region of human Heparanase 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab59787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 57 kDa (predicted molecular weight: 62 kDa).

Target

  • Function
    Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Also implicated in the extravasation of leukocytes and tumor cell lines. Due to its contribution to metastasis and angiogenesis, it is considered to be a potential target for anti-cancer therapies.
  • Tissue specificity
    Highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 79 family.
  • Post-translational
    modifications
    Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, 8 kDa and 50 kDa product. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme.
    N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility.
  • Cellular localization
    Lysosome membrane. Secreted. Secreted, internalised and transferred to late endosomes/lysosomes as a proheparanase. In lysosomes, it is processed into the active form, the heparanase. The uptake or internalisation of proheparanase is mediated by HSPGs. Heparin appears to be a competitor and retain proheparanase in the extracellular medium.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endo glucoronidase antibody
    • Endo-glucoronidase antibody
    • HEP antibody
    • Heparanase 50 kDa subunit antibody
    • Heparanase antibody
    • Heparanase-1 antibody
    • Heparanase1 antibody
    • Hpa 1 antibody
    • HPA antibody
    • Hpa1 antibody
    • HPR 1 antibody
    • HPR1 antibody
    • HPSE 1 antibody
    • HPSE antibody
    • HPSE_HUMAN antibody
    • HPSE1 antibody
    • HSE 1 antibody
    • HSE1 antibody
    see all

Images

  • All lanes : Anti-Heparanase 1 antibody (ab59787) at 1/1000 dilution

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 62 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)
  • Anti-Heparanase 1 antibody (ab59787) + Human epidermis

    Predicted band size : 62 kDa
    Observed band size : 8,50 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Cui H  et al. Heparanase enhances nerve-growth-factor-induced PC12 cell neuritogenesis via the p38 MAPK pathway. Biochem J 440:273-82 (2011). Read more (PubMed: 21831044) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry
Sample
Rat Cell (Sciatic nerve)
Permeabilization
No
Specification
Sciatic nerve
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 28 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up