Overview

  • Product name
    Anti-Heparanase 1 antibody
    See all Heparanase 1 primary antibodies
  • Description
    Rabbit polyclonal to Heparanase 1
  • Specificity
    ab85543 recognizes the 65kD precursor as well as the 50kD and 8kD subunits of Heparanase 1.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Human 50kD-8kD Heparanase 1 heterodimer.

  • Positive control
    • Spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab85543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 62 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Also implicated in the extravasation of leukocytes and tumor cell lines. Due to its contribution to metastasis and angiogenesis, it is considered to be a potential target for anti-cancer therapies.
  • Tissue specificity
    Highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 79 family.
  • Post-translational
    modifications
    Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, 8 kDa and 50 kDa product. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme.
    N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility.
  • Cellular localization
    Lysosome membrane. Secreted. Secreted, internalised and transferred to late endosomes/lysosomes as a proheparanase. In lysosomes, it is processed into the active form, the heparanase. The uptake or internalisation of proheparanase is mediated by HSPGs. Heparin appears to be a competitor and retain proheparanase in the extracellular medium.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endo glucoronidase antibody
    • Endo-glucoronidase antibody
    • HEP antibody
    • Heparanase 50 kDa subunit antibody
    • Heparanase antibody
    • Heparanase-1 antibody
    • Heparanase1 antibody
    • Hpa 1 antibody
    • HPA antibody
    • Hpa1 antibody
    • HPR 1 antibody
    • HPR1 antibody
    • HPSE 1 antibody
    • HPSE antibody
    • HPSE_HUMAN antibody
    • HPSE1 antibody
    • HSE 1 antibody
    • HSE1 antibody
    see all

Anti-Heparanase 1 antibody images

  • ab85543 staining Heparanase 1 in Human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% NHS for 30 minutes at room temperature; antigen retrieval was by heat mediation with an EDTA buffer. Samples were incubated with primary antibody (1/4000 in NHS) for 8 hours. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview



  • Predicted band size : 62 kDa
    Observed band size : 62 kDa
    Purified recombinant human heparanase served as a control (Lane1). Lane2: An extract from 6.5x10e3 CHO cells, transfected with human Heparanase; Lane3: extract from 8x10e4 CHO cells, transfected with mouse Heparanase, and Lane4: extract from 5x10e5 non-transfected CHO dhfr- cells, were loaded onto 4-12% NuPage gel. The proteins were transferred to a PVDF and subjected to Western Blot analysis using ab85543 at 1:1000 dilution. The 65kD precursor and the 50kD and 8kD subunits are clearly detected. Lane 1 : Purified recombinant human Heparanase served as a control
    Lane 2 : Extract from 6.5x10e3 CHO cells, transfected with human Heparanase
    Lane 3 : Extract from 8x10e4 CHO cells, transfected with mouse Heparanase
    Lane 4 : Extract from 5x10e5 non-transfected CHO dhfr- cells

    Cell were loaded onto 4-12% NuPage gel. The proteins were transferred to a PVDF and subjected to Western Blot analysis using ab85543 at 1:1000 dilution. The cleavage of the 65 kDa form leads

References for Anti-Heparanase 1 antibody (ab85543)

This product has been referenced in:
  • Zhang Y  et al. Elemene inhibits the migration and invasion of 4T1 murine breast cancer cells via heparanase. Mol Med Rep 16:794-800 (2017). Read more (PubMed: 28560389) »
  • An X  et al. Hyperoside pre-treatment prevents glomerular basement membrane damage in diabetic nephropathy by inhibiting podocyte heparanase expression. Sci Rep 7:6413 (2017). Read more (PubMed: 28743882) »

See all 4 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA-MB-231)
Permeabilization
Yes - Triton X-100 0.1%
Specification
MDA-MB-231
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 20 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung)
Specification
Lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
No
Blocking step
NHS as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Dr. Jim Manavis

Verified customer

Submitted Nov 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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