The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
FunctionThrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils.
Tissue specificityExpressed predominantly in liver. Also present in plasma.
Involvement in diseaseThrombophilia due to heparin cofactor 2 deficiency
Sequence similaritiesBelongs to the serpin family.
DomainThe N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition.
Post-translational modificationsPhosphorylation sites are present in the extracellular medium.