Anti-Hepatitis A virus 2C antibody [1] (ab81140)

Overview

  • Product nameAnti-Hepatitis A virus 2C antibody [1]
    See all Hepatitis A virus 2C primary antibodies
  • Description
    Mouse monoclonal [1] to Hepatitis A virus 2C
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with Recombinant Fragment. Predicted to react with Hepatitis A virus.
  • Immunogen

    Recombinant Protein 2C fragment, corresponding to amino acids 1141-1370 of the polyprotein from Hepatitis A virus strain H2K20, expressed in E.coli.

  • Positive control
    • Recombinant Hepatitis A virus 2C fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab81140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    WB: 1/1000. Detects a band of approximately 35 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceHepatitis A Virus (HAV) is a 27nm nonenveloped, spherical, positive stranded RNA virus, classified within the genus hepatovirus of the picornavirus family and is among the smallest and structurally simplest of the RNA animal viruses. A single large polyprotein is expressed from a large open reading frame extending through most of the genomic RNA. This polyprotein is subsequently cleaved by a viral protease (3Cpro) to form capsid proteins and several nonstructural proteins. Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities.

    Anti-Hepatitis A virus 2C antibody [1] images

    • All lanes : Anti-Hepatitis A virus 2C antibody [1] (ab81140) at 1/1000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Recombinant Hepatitis A virus 2C fragment


      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    References for Anti-Hepatitis A virus 2C antibody [1] (ab81140)

    ab81140 has not yet been referenced specifically in any publications.

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