The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 35 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceHepatitis A Virus (HAV) is a 27nm nonenveloped, spherical, positive stranded RNA virus, classified within the genus hepatovirus of the picornavirus family and is among the smallest and structurally simplest of the RNA animal viruses. A single large polyprotein is expressed from a large open reading frame extending through most of the genomic RNA. This polyprotein is subsequently cleaved by a viral protease (3Cpro) to form capsid proteins and several nonstructural proteins. Protein 2C associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities.
Anti-Hepatitis A virus 2C antibody  images
Western blot - Hepatitis A H2K20 antibody  (ab81140)
All lanes : Anti-Hepatitis A virus 2C antibody  (ab81140) at 1/1000 dilution
Lane 1 : Molecular weight markers Lane 2 : Recombinant
Hepatitis A virus 2C fragment