Anti-Hepatitis B Virus Core Antigen antibody [14E11] (ab8638)
Key features and details
- Mouse monoclonal [14E11] to Hepatitis B Virus Core Antigen
- Suitable for: ELISA, IHC-FoFr, IHC-Fr, ICC/IF, IP, IHC-P, WB
- Reacts with: Hepatitis B virus
- Isotype: IgG2b
Overview
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Product name
Anti-Hepatitis B Virus Core Antigen antibody [14E11]
See all Hepatitis B Virus Core Antigen primary antibodies -
Description
Mouse monoclonal [14E11] to Hepatitis B Virus Core Antigen -
Host species
Mouse -
Specificity
ab8638 was raised against serotype ayw but will work with all other genotypes.
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Tested applications
Suitable for: ELISA, IHC-FoFr, IHC-Fr, ICC/IF, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Hepatitis B virus -
Immunogen
Tissue, cells or virus corresponding to Hepatitis B Virus Core Antigen. Purified Denatured Hepatitis B Core Antigen
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Epitope
135-141aa (PNAPILS) -
General notes
This product is raised in the same, but denatured, HBcAg protein sequence as ab8637, hence have more robust activity in denaturing western blots/IF etc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
14E11 -
Myeloma
Sp2/0 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-FoFr |
1/100.
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IHC-Fr |
1/100.
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ICC/IF |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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IHC-P |
1/100.
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WB |
1/1000.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-FoFr
1/100. |
IHC-Fr
1/100. |
ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-P
1/100. |
WB
1/1000. |
Target
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Relevance
Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection. -
Cellular localization
Capsid protein: Virion. Host cytoplasm, hepatocyte nucleus. -
Database links
- Entrez Gene: 2828293 Hepatitis B virus
- Entrez Gene: 944568 Hepatitis B virus
- SwissProt: P03146 Hepatitis B virus
- SwissProt: P03147 Hepatitis B virus
- SwissProt: P03148 Hepatitis B virus
- SwissProt: P0C573 Hepatitis B virus
- SwissProt: P69706 Hepatitis B virus
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Alternative names
- C antibody
- Capsid protein antibody
- Core and e antigen antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (14)
ab8638 has been referenced in 14 publications.
- Komatsu H et al. Signature of chronic hepatitis B virus infection in nails and hair. BMC Infect Dis 22:431 (2022). PubMed: 35509029
- Oikawa R et al. DNA methylation at hepatitis B virus integrants and flanking host mitochondrially encoded cytochrome C oxidase III. Oncol Lett 24:424 (2022). PubMed: 36284651
- Colant N et al. Escherichia Coli-Based Cell-Free Protein Synthesis for Iterative Design of Tandem-Core Virus-Like Particles. Vaccines (Basel) 9:N/A (2021). PubMed: 33669126
- Lucifora J et al. Evidence for long-term association of virion-delivered HBV core protein with cccDNA independently of viral protein production. JHEP Rep 3:100330 (2021). PubMed: 34409278
- Ma A et al. A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B. Hum Vaccin Immunother 16:756-778 (2020). PubMed: 31687879