Reacts with Hepatitis type B virus.
Not yet tested in other species.
HBcAg purified from the lysates of E.coli infected with the viral core DNA.
Liver infected with the Hepatitis type B virus.
This antibody detectes the antigen in the nuclei of the liver cells of the Hepatitis type B virus infected patients. However, occasional staining may also be seen in the perinuclear cytoplasm of the infected cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Primary antibody notesThis antibody detectes the antigen in the nuclei of the liver cells of the Hepatitis type B virus infected patients. However, occasional staining may also be seen in the perinuclear cytoplasm of the infected cells.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/150.
An incubation period of 30 minutes at room temperature is recommended.
RelevanceHepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection.
The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.