Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab8637)


  • Product nameAnti-Hepatitis B Virus Core Antigen antibody [C1]
    See all Hepatitis B Virus Core Antigen primary antibodies
  • Description
    Mouse monoclonal [C1] to Hepatitis B Virus Core Antigen
  • SpecificityThis antibody reacts with HBV Core Antigen (Major antigenic determinant, c1). Ab8637 should recognize both the precoreprotein and core protein. It will not recognize the precorprotein under native conditions, because this protein can not self assemble into particles.
  • Tested applicationsSuitable for: ELISA, IHC-Fr, IP, WB, ICC/IFmore details
  • Species reactivity
    Has been tested in ADW Hep B subtype.
  • Immunogen

    Purified Hepatitis B Core Antigen

  • EpitopeAround aa positions 70-80, major, or "c1" determinant



Our Abpromise guarantee covers the use of ab8637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr 1/100.
IP Use at an assay dependent concentration.
WB 1/1000. We recommend boiling the sample for 10 minutes before loading.
ICC/IF Use at an assay dependent concentration.


  • RelevanceHepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.
  • Cellular localizationCapsid protein: Virion. Host cytoplasm, hepatocyte nucleus.
  • Alternative names
    • C antibody
    • Capsid protein antibody
    • Core and e antigen antibody
    • Core antigen antibody
    • Core protein antibody
    • HBc antibody
    • HBcAg antibody
    • HBVgp4 antibody
    • Hepatitis B Virus core antigen antibody
    • p21.5 antibody
    • precore/core protein antibody
    see all

Anti-Hepatitis B Virus Core Antigen antibody [C1] images

  • Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg(full length, 1-183

References for Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab8637)

This product has been referenced in:
  • Hasmoni SS  et al. Detection and precipitation of hepatitis B core antigen using a fusion bacteriophage. J Gen Appl Microbiol 51:125-31 (2005). Read more (PubMed: 15942873) »
  • Preikschat P  et al. Expression, assembly competence and antigenic properties of hepatitis B virus core gene deletion variants from infected liver cells. J Gen Virol 80 ( Pt 7):1777-88 (1999). Read more (PubMed: 10423147) »

See all 3 Publications for this product

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If there is anything else I can help you with, please let me know.

Thank you for contacting Abcam regarding ab8637.

I am sorry that you are experiencing difficulties with this antibody in WB. In order to assist you further, I wanted to clarify several points in your protocol.

You mention th...

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Thank you for your reply.

Upon further investigation, this target contains several phosphorylation sites which may account for the shift in molecular weight. Otherwise, I cannot account for the shift.

If you would like to try anot...

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Thank you for contacting Abcam regarding ab8637. I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information and image you provided and would like to ask some additional questions. ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Huh7 Human Hepatoma cells)
Specification Huh7 Human Hepatoma cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Saponin

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Verified customer

Submitted Nov 23 2010

Thank you for your question. I can confirm that this Hepatitis B Virus Core Antigen antibody, ab8637, will recognize native core particles but not native e antigen. Under denaturing conditions, it willr ecognize all core polypeptides, containing ep...

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Ab8637 and ab8639 should recognize the precorerotein in WB but there is no "cross-reaction" as all proteins are distinguishable by mobility on the gel (i.e they will recognise both the precoreprotein and core protein). AB8638 may or may not recogni...

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Here is the information that I could obtain regarding ab8639, ab8638, and ab8637. The recommended starting dilutions are 1:1,000 and down in 10-fold increments. As for detection, any known detection method would work. Once again, those mabs had being c...

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I have received the following information regarding ab8255 and ab2045, but will have to get back to you about the other antibodies. The originator has said that they tested the antibodies as pairs in internal ELISA. Possible pairs are H6F5 (ab2045) - H...

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Thank you for your enquiry. I was able to obtain the following information regarding the use of these antibodies in ELISA. Ab8255 and Ab2045: These antibodies have been tested in ELISA with the native antigen. The antibodies can be used as a pair in...

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